Golestan University of Medical Sciences Repository

Combination of gold nanoparticles with low-LET irradiation: an approach to enhance DNA DSB induction in HT29 colorectal cancer stem-like cells

Abbasian, M. and Baharlouei, A. and Arab-Bafrani, Z. and Lightfoot, D.A. (2019) Combination of gold nanoparticles with low-LET irradiation: an approach to enhance DNA DSB induction in HT29 colorectal cancer stem-like cells. Journal of Cancer Research and Clinical Oncology, 145 (1). pp. 97-107.

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Purpose: High-linear energy transfer (high LET) irradiation has significant cytotoxic effects on different cancerous stem-like cells (CSLCs) such as colorectal CSLCs. A review of the literature has indicated that the presence of gold nanoparticles (GNPs) enables low-LET irradiation to produce highly non-homogeneous dose distributions like high-LET irradiation. The purpose of this study was to evaluate the radioresponsiveness of HT29 colorectal CSLCs under low-LET irradiation (X-ray) and in the presence of GNPs. Methods: Radioresponsiveness was evaluated using the �-H2AX foci formation assay, the clonogenic assay, the cell cycle progression assay and analyses of radiobiological parameters. Results: In the presence of GNPs, the survival fraction of HT29 CSLCs was significantly reduced and caused significant changes in the radiobiological parameters after irradiation. In addition, �-H2AX assay showed that in the presence of GNPs, the persistent DNA double-strand breaks were significantly increased in irradiated HT29 CSLCs. The relative biological effectiveness value of GNPs with X-rays was about 1.6 for HT-29 CSLCs at the 10 of cell survival fraction (D 10 level) when compared to X-rays alone. Conclusion: Therefore, the combination of GNPs with X-ray irradiation has the potential to kill HT29 CSLCs greater than the X-ray alone, and may be considered as an alternative for high-LET irradiation. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: CD133 antigen; gold nanoparticle; histone H2AX; DNA; gold; H2AFX protein, human; histone; metal nanoparticle, Article; cancer stem cell; cancer stem like cell; cell cycle progression; cell survival; clonogenic assay; colorectal cancer; controlled study; cytotoxicity; double stranded DNA break; histone phosphorylation; HT-29 cell line; human; human cell; linear energy transfer; priority journal; protein expression; radiation dose distribution; radiosensitivity; relative biologic effectiveness; X irradiation; cancer stem cell; cell cycle; chemistry; colorectal tumor; HT-29 cell line; metabolism; pathology; radiation response; real time polymerase chain reaction; reverse transcription polymerase chain reaction; serum-free medium; upregulation; X ray, Cell Cycle; Cell Survival; Colorectal Neoplasms; Culture Media, Serum-Free; DNA; DNA Breaks, Double-Stranded; Dose-Response Relationship, Radiation; Gold; Histones; HT29 Cells; Humans; Metal Nanoparticles; Neoplastic Stem Cells; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Up-Regulation; X-Rays
Subjects: QTفیزیولوژی
آسیب شناسی QZ
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 14 Apr 2019 04:25
Last Modified: 14 Apr 2019 04:25
URI: http://eprints.goums.ac.ir/id/eprint/9905

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