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Test bacterial inclusion body for activity prior to start denaturing and refolding processes to obtain active eukaryotic proteins

Hamidi, S.R. and Safdari, Y. and Sheikh Arabi, M. (2019) Test bacterial inclusion body for activity prior to start denaturing and refolding processes to obtain active eukaryotic proteins. Protein Expression and Purification, 154. pp. 147-151.

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Abstract

One of a major drawbacks correlated with expressing antibody fragments in bacterial cells is insolubility, which is often regarded as an obstacle in obtaining active molecules. Recombinant proteins aggregated as inclusion bodies within bacterial cells are thought to be unfolded or misfolded, and therefore inactive. So, denaturing and refolding strategies, which are laborious and sometime inefficient, are used to obtain correctly-folded active proteins. In the current study, we show that large quantities of correctly folded and completely active scFv molecules are there in bacterial inclusion bodies; they only need to be isolated from inclusion bodies. © 2018 Elsevier Inc.

Item Type: Article
Additional Information: cited By 0
Subjects: QU بیوشیمی
میکروب شناسی وایمنی شناسی QW
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 13 Apr 2019 07:51
Last Modified: 13 Apr 2019 07:51
URI: http://eprints.goums.ac.ir/id/eprint/9879

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