Golestan University of Medical Sciences Repository

Potent in vitro antileishmanial activity of a nanoformulation of cisplatin with carbon nanotubes against Leishmania major

Akhtari, J. and Faridnia, R. and Kalani, H. and Bastani, R. and Fakhar, M. and Rezvan, H. and Beydokhti, A.K. (2019) Potent in vitro antileishmanial activity of a nanoformulation of cisplatin with carbon nanotubes against Leishmania major. Journal of Global Antimicrobial Resistance, 16. pp. 11-16.

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Abstract

Objectives: The aim of this study was to evaluate the cytotoxicity and antileishmanial activity of cisplatin-bonded carbon nanotubes both against promastigotes and amastigotes of Leishmania major in vitro. Methods: Cisplatin-bonded single-walled carbon nanotubes (CP-SWCNT) and cisplatin-bonded multi-walled carbon nanotubes (CP-MWCNT) were considered as test compounds. In addition, SWCNT, MWCNT, free cisplatin and meglumine antimoniate (Glucantime ® ) were considered as controls. The effect of each compound was evaluated both on promastigote and amastigote stages of L. major and the results were compared. Results: There was a statistically significant difference between the half-maximal inhibitory concentration (IC 50 ) of CP-SWCNT and each of the controls, including SWCNT, cisplatin and Glucantime ® (P < 0.05). In addition, IC 50 values of CP-MWCNT and each of the controls, including MWCNT, cisplatin and Glucantime ® , were significantly different both for promastigotes and amastigotes (P < 0.05). However, the selectivity index (SI) of CP-SWCNT was <10 (5.23), indicating that this compound is not completely safe. Moreover, the SI values of CP-MWCNT (12.54) and Glucantime ® (16.28) were >10, indicating the selective effect of these two compounds on the parasite. Moreover, the IC 50 of CP-MWCNT (0.11 ± 0.09 μM) for amastigotes was 41-fold lower than that of Glucantime ® (4.52 ± 1.31 μM), suggesting that a lower dose of CP-MWCNT in comparison with Glucantime ® is required to kill 50 of amastigotes. Conclusions: According to the potent in vitro antileishmanial activity of CP-MWCNT at low concentration against L. major, we suggest that they are evaluated in an animal model. © 2018 International Society for Chemotherapy of Infection and Cancer

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: antileishmanial agent; carbon nanotube; cisplatin; meglumine antimonate; multi walled nanotube; single walled nanotube, amastigote; animal cell; Article; controlled study; cytotoxicity; IC50; infrared radiation; Leishmania major; mouse; nonhuman; peritoneum macrophage; priority journal; promastigote; selectivity index; transmission electron microscopy
Subjects: بیماریهای پوست WR
فارماکولوژی QV
> دانشکده داروسازی > فارماکولوژی QV
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 13 Apr 2019 05:30
Last Modified: 13 Apr 2019 05:30
URI: http://eprints.goums.ac.ir/id/eprint/9842

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