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α2-Adrenoceptor-ir neurons’ density changes after single dose of clonidine and yohimbine administration in the hippocampus of male rat

Jahanshahi, M. and Nikmahzar, E. and Elyasi, L. and Babakordi, F. and Hooshmand, E. (2018) α2-Adrenoceptor-ir neurons’ density changes after single dose of clonidine and yohimbine administration in the hippocampus of male rat. International Journal of Neuroscience, 128 (5). pp. 404-411.

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Abstract

Objective: Despite the important role of α2-adrenoceptors in pain modulation processes, the impact of administration of α2-adrenoceptor agonist and antagonist on the density of hippocampal α2-adrenoceptor-immunoreactive neurons has not been investigated. Therefore, we aimed to determine the effect of single doses of clonidine and yohimbine on the density of α2-adrenoceptor-immunoreactive neurons in rat hippocampus. Materials and Methods: Adult male Wistar rats received a single dose of clonidine (0.7 mg/kg) alone or 5 min after intraperitoneal (1 mg/kg) and/or intracerebroventricular (5 µg/kg) injection of yohimbine. After histological processing, neurons with α2-adrenoceptor immunoreactivity were identified and counted through immunohistochemical analysis of hippocampal regions. Results: Clonidine slightly increased the number of α2-adrenoceptor-immunoreactive neurons in the hippocampal subregions compared with the normal saline group. Intraperitoneal injection of yohimbine followed by injection of clonidine significantly increased the number of α2-adrenoceptor-immunoreactive neurons in subregions cornu ammonis 1 (CA1) and cornu ammonis 3 (CA3). Intracerebroventricular injection of yohimbine after injection of clonidine significantly reduced the number of α2-adrenoceptor-immunoreactive neurons in all hippocampal subregions. Conclusion: The present study demonstrates that intraperitoneal administration of α2-adrenoceptor agonist clonidine increases the density of α2-adrenoceptor-immunoreactive neurons in rat hippocampus, while intracerebroventricular injection of yohimbine decreases the density of these neurons. © 2017 Informa UK Limited, trading as Taylor & Francis Group.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: alpha 2 adrenergic receptor; clonidine; yohimbine; adrenergic receptor stimulating agent; alpha 2 adrenergic receptor; clonidine; yohimbine, adult; animal cell; animal experiment; animal tissue; Article; cell density; controlled study; dentate gyrus; drug mechanism; granular cell; hippocampal CA1 region; hippocampal CA3 region; hippocampus; immunohistochemistry; immunoreactivity; male; nerve cell; nonhuman; protein expression; pyramidal nerve cell; rat; single drug dose; adrenergic nerve cell; analysis of variance; animal; cell count; cytology; dose response; drug administration route; drug effect; metabolism; Wistar rat, Adrenergic Agents; Adrenergic Neurons; Analysis of Variance; Animals; Cell Count; Clonidine; Dose-Response Relationship, Drug; Drug Administration Routes; Hippocampus; Male; Rats; Rats, Wistar; Receptors, Adrenergic, alpha-2; Yohimbine
Subjects: سیستم عصبی WL
QS آناتومی انسان
QTفیزیولوژی
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 24 Dec 2018 05:53
Last Modified: 24 Dec 2018 05:53
URI: http://eprints.goums.ac.ir/id/eprint/9752

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