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Gene silencing of TGFβRII can inhibit glioblastoma cell growth

Aval, F.O. and Amiri, S.A. and Azadmehr, A. and Oladnabi, M. and Saadat, P. and Ebrahimi, H. and Baradaran, B. and Mansoori, B. and Pourabdolhossein, F. and Torabian, P. and Hajiahmadi, M. (2018) Gene silencing of TGFβRII can inhibit glioblastoma cell growth. Asian Pacific Journal of Cancer Prevention, 19 (9). pp. 2681-2686.

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Abstract

Objective: Glioblastoma (GBM) is the most malignant and aggressive type of glioma, associated with a high rate of mortality. The transforming growth factor-β receptor II (TGFβ RII) is involved in glioma initiation and progression. On the other hand, TGFβ RII silencing is critical to the inhibition of GBM. Therefore, we aimed to determine the effects of specific TGFβ RII siRNA on the survival of U-373MG cells. Methods: TGFβ RII siRNA was transfected, and qRT-PCR was performed to examine TGFβ RII mRNA expression. Cell survival was determined using colorimetric MTT assay, and platelet-derived growth factor-BB (PDGF-BB) level was measured in the culture supernatant using ELISA assay. Result: Our findings indicated that specific siRNAs could dose-dependently suppress TGFβ RII mRNA expression after 48 hours. In addition, treatment with TGFβ RII siRNA significantly reduced tumor cell survival and decreased the amount of PDGF-BB protein in the cell culture supernatant. Conclusion: Our results suggest that TGFβ RII silencing can be a promising complementary treatment for glioma. © 2018, Asian Pacific Organization for Cancer Prevention.

Item Type: Article
Additional Information: cited By 0
Subjects: سیستم عصبی WL
آسیب شناسی QZ
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 23 Dec 2018 10:32
Last Modified: 23 Dec 2018 10:32
URI: http://eprints.goums.ac.ir/id/eprint/9740

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