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Cardioprotective and anti-apoptotic effects of Potentilla reptans L. root via Nrf2 pathway in an isolated rat heart ischemia/reperfusion model

Enayati, A. and Yassa, N. and Mazaheri, Z. and Rajaei, M. and Pourabouk, M. and Ghorghanlu, S. and Basiri, S. and Khori, V. (2018) Cardioprotective and anti-apoptotic effects of Potentilla reptans L. root via Nrf2 pathway in an isolated rat heart ischemia/reperfusion model. Life Sciences, 215. pp. 216-226.

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Abstract

Background: Previous studies have shown that proanthocyanidins have cardioprotective effects which are mediated via the release of nitric oxide (NO) ultimately resulting in increasing the antioxidant activity. We have investigated to show whether 1) the total extract and ethyl acetate fraction (Et) of Potentilla reptans root have an ischemic preconditioning (IPC) effect, 2) P. reptans has antioxidant and cardioprotective effects mediated by nuclear factor erythroid 2-related factor 2 (Nrf2) pathway and scavenging of reactive oxygen species (ROS), 3) NO, caspase-3 and Bcl-2/Bax are involved in the IPC effect of P. reptans. Methods: Male Wistar rats were divided into 10 groups. The isolated hearts were subjected to 30 min of ischemia and 100 min of reperfusion. The P. reptans was applied before the main ischemia. The infarct size was estimated by triphenyl-tetrazolium chloride staining. The hemodynamic parameters and ventricular arrhythmias were calculated during the reperfusion. Antioxidant markers and immunohistochemistry assays were determined at the end of the protocol. Results: The Et significantly decreased the infarct size, arrhythmia scores, ventricular fibrillation incidence, and enhanced the hemodynamic parameters in a concentration-dependent manner against the ischemia/reperfusion group. SOD and CAT activity were increased and MDA level was decreased in response to the Et. Meanwhile, Et attenuated the suppression of Nrf2 expression and reduced the apoptotic indexes. The cardioprotective effect of P. reptans was abrogated by L-NAME. Conclusions: P. reptans demonstrated that the cardioprotective preconditioning effects via NO release, Nrf2 pathway, and antioxidant activity lead to a decrease in the apoptotic index. © 2018

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: acetic acid ethyl ester; caspase 3; catalase; n(g) nitroarginine methyl ester; nitric oxide; plant extract; Potentilla reptans extract; proanthocyanidin; protein Bax; protein bcl 2; reactive oxygen metabolite; superoxide dismutase; transcription factor Nrf2; triphenyltetrazolium; unclassified drug, animal experiment; animal model; antioxidant activity; apoptosis; Article; controlled study; enzyme activity; heart infarction size; heart muscle ischemia; heart protection; heart ventricle arrhythmia; heart ventricle fibrillation; hemodynamic parameters; immunohistochemistry; incidence; ischemic preconditioning; male; medicinal plant; nonhuman; plant root; Potentilla reptans; rat; reperfusion; reperfusion injury
Subjects: سیستم قلب وعروق WG
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 18 Dec 2018 06:14
Last Modified: 18 Dec 2018 06:14
URI: http://eprints.goums.ac.ir/id/eprint/9706

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