Golestan University of Medical Sciences Repository

Association of tumor necrosis factor-alpha gene promoter polymorphism and its mRNA expression level in coronary artery disease

Nejati, P. and Naeimipour, S. and Salehi, A. and Shahbazi, M. (2018) Association of tumor necrosis factor-alpha gene promoter polymorphism and its mRNA expression level in coronary artery disease. Meta Gene, 18. pp. 122-126.

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Abstract

Background: umor necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine that may play an important role in inflammatory diseases like coronary artery disease (CAD). As a result of controversy on the impact of polymorphisms in the promoter region of TNF-α gene on transcription, this case control study was designed to evaluate the effects of TNF-α -308G > A (rs1800629) and TNF-α − 238G > A (rs361525) on mRNA expression level and risk of CAD. Methods: Overall, 379 patients with CAD and 461 healthy controls participated in this study. Subjects were matched in terms of age, sex and ethnic origin. Genotyping was performed using single specific primer-polymerase chain reaction (SSP-PCR) and TNF-α mRNA expression analysis was carried out by quantitative real time PCR. Results: A/A and A/G genotypes in TNF-α (−308G > A) gene were significantly more frequent among patients than controls. The TNF-α (−238G > A) did not differ between CAD patients and controls (the TNF-α (−238G > A) genotypes and allele frequency showed no significant differences). Combination of AA TNF-α (−308) /GG TNF-α (−238) and AG TNF-α (−308) /AG TNF-α (−238) genotypes demonstrated higher frequencies in patients than in controls (P <.05). TNF-α mRNA expression level showed significantly higher in patients carrying A/A genotype at TNF-α (−308) compared to healthy individuals. Conclusion: Although there was no significant association between TNF-α -238 G > A polymorphism with TNF-α expression level and pathogenesis of CAD in the studied population, The TNF-α (−308G > A) gene polymorphism was associated with its expression and development of CAD. © 2018

Item Type: Article
Additional Information: cited By 0
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 29 Sep 2018 08:05
Last Modified: 29 Sep 2018 08:05
URI: http://eprints.goums.ac.ir/id/eprint/9647

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