Golestan University of Medical Sciences Repository

Metallo‑β‑lactamase‑mediated resistance among clinical carbapenem‑resistant Pseudomonas aeruginosa isolates in northern Iran: A potential threat to clinical therapeutics

Dogonchi, A.A. and Ghaemi, E.A. and Ardebili, A. and Yazdansetad, S. and Pournajaf, A. (2018) Metallo‑β‑lactamase‑mediated resistance among clinical carbapenem‑resistant Pseudomonas aeruginosa isolates in northern Iran: A potential threat to clinical therapeutics. Tzu Chi Medical Journal, 30 (2). pp. 90-96.

Full text not available from this repository.

Abstract

Objective: Carbapenems are effective agents to treat multidrug‑resistant (MDR) strains of bacteria, including Pseudomonas aeruginosa. However, there is a potential threat of emergence of carbapenem‑resistant P. aeruginosa (CRPA). The aim of this study was to determine antibiotic susceptibility patterns and metallo‑beta‑lactamase (MBL)‑mediated resistance in clinical P. aeruginosa isolates. Materials and Methods: Different clinical specimens were subjected to conventional culture‑based identification of P. aeruginosa. Antimicrobial susceptibility patterns and MBL production were evaluated using the Kirby‑Bauer and combined double‑disk synergy test methods, respectively. Multiplex polymerase chain reaction was performed to investigate the presence of the blaIMP, blaVIM, blaNDM, blaSPM, and blaSIM genes. Results: A total of 71 clinical P. aeruginosa isolates were recovered, of which 28.17 were identified as CRPA. The most active antibiotics were colistin and polymyxin B (92.96 susceptibility to each). A total of 35 and 50 of CRPA isolates were MDR and extensively drug‑resistant (XDR), respectively. MBL activity was shown in 20 of CRPA. A total of 90, 40, and 5 of CRPA isolates harbored the blaIMP, blaVIM, and blaNDM genes, respectively. No correlation was found between the MBL‑encoding genes of P. aeruginosa and patient characteristics. Conclusion: Although the prevalence of CRPA in our therapeutic centers was relatively low, this rate of carbapenem resistance reflects a threat limiting treatment choices. A high prevalence of MDR/XDR phenotypes among the MBL‑producer isolates suggests the need for continuous assessment of antimicrobial susceptibility and surveillance of antibiotic prescription. In addition, infection control measures are needed to prevent further dissemination of these organisms. © 2018 Tzu Chi Medical Journal | Published by Wolters Kluwer - Medknow.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: amikacin; ceftazidime; ciprofloxacin; colistin; gentamicin; imipenem; levofloxacin; meropenem; metallo beta lactamase; piperacillin plus tazobactam; polymyxin B; tobramycin, adolescent; adult; age distribution; aged; antibiotic resistance; Article; bacterium isolate; blood; carbapenem resistant Pseudomonas aeruginosa; child; comparative study; controlled study; ear; female; hospital patient; human; Iran; major clinical study; male; multiplex polymerase chain reaction; nonhuman; preschool child; prevalence; Pseudomonas aeruginosa; respiratory system; school child; urine; very elderly; wound; young adult
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 29 Sep 2018 07:01
Last Modified: 29 Sep 2018 07:01
URI: http://eprints.goums.ac.ir/id/eprint/9630

Actions (login required)

View Item View Item