Golestan University of Medical Sciences Repository

Development of a novel explant culture method for the isolation of mesenchymal stem cells from human breast tumor

Sineh Sepehr, K. and Razavi, A. and Saeidi, M. and Mossahebi-Mohammadi, M. and Abdollahpour-Alitappeh, M. and Hashemi, S.M. (2018) Development of a novel explant culture method for the isolation of mesenchymal stem cells from human breast tumor. Journal of Immunoassay and Immunochemistry. pp. 1-11.

Full text not available from this repository.

Abstract

Background: Mesenchymal stem cells (MSCs) were isolated from various sources, including various types of tumors. However choosing an appropriate isolation method is an important step in obtaining cells with optimal quality and yield in companion with economical considerations. The purpose of this study was to isolate more pure MSCs from human breast tumor tissue by a modified explant culture method. Methods and Materials: The tumor tissues (n = 8) were cut into 1 to 3-mm cube-like pieces (explant). Each explant was placed in a well of 24-well format plates, cultured in Dulbecco’s Modified Eagle’s medium (DMEM), and maintained at 37°C with 5 humidified incubator. Morphological phenotypes of the cells were surveyed by an inverted microscope and wells with rather homogenous fibroblast-like morphology cell were considered as positive and selected for more expansion and characterization. Results: A total of 185 wells, 63.7 of wells were positive that were chosen for expansion. Flowcytometry analysis demonstrated that isolated cells were positive for CD73, CD44, CD29, CD105, and CD90 but negative for CD11b, CD45, CD34, and HLA‑DR. In addition, cells possessed the capability of multipotential differentiation into osteoblasts and adipocytes. © 2018 Taylor & Francis

Item Type: Article
Additional Information: cited By 0; Article in Press
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 26 Jun 2018 08:16
Last Modified: 26 Jun 2018 08:16
URI: http://eprints.goums.ac.ir/id/eprint/9600

Actions (login required)

View Item View Item