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Molecular identification and antifungal susceptibility pattern of non-albicans candida species isolated from vulvovaginal candidiasis

Abbasi Nejat, Z. and Farahyar, S. and Falahati, M. and Khozani, M.A. and Hosseini, A.F. and Faiazy, A. and Ekhtiari, M. and Hashemi-Hafshenjani, S. (2018) Molecular identification and antifungal susceptibility pattern of non-albicans candida species isolated from vulvovaginal candidiasis. Iranian Biomedical Journal, 22 (1). pp. 33-41.

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Abstract

Background: Vulvovaginal candidiasis (VVC) is an important health problem caused by Candida spp. The aim of this study was molecular identification, phylogenetic analysis, and evaluation of antifungal susceptibility of nonalbicans Candida isolates from VVC. Methods: Vaginal secretion samples were collected from 550 vaginitis patients at Sayyad Shirazi Medical and Educational Center of Gorgan (Golestan Province, Iran) from May to October 2015. Samples were analyzed using conventional mycological and molecular approaches. Clinical isolates were analyzed with specific PCR using CGL primers, and the internal transcribed spacer region and the D1-D2 domain of the large-subunit rRNA gene were amplified and sequenced. Susceptibility to amphotericin B, fluconazole, itraconazole, and clotrimazole was determined by the guidelines of the Clinical and Laboratory Standard Institute. Results: In total, 35 non-albicans Candida isolates were identified from VVC patients. The isolates included 27 strains of Candida glabrata (77.1), 5 Candida krusei (Pichia kudriavzevii; 14.3), 2 Candida kefyr (Kluyveromyces marxianus; 5.7), and 1 Candida lusitaniae (Clavispora lusitaniae; 2.9). The fungicides itraconazole and amphotericin B were effective against all species. One isolate of C. glabrata showed resistance to fluconazole and clotrimazole, and 26 isolates of C. glabrata indicated dose-dependent susceptibility to fluconazole. C. lusitaniae was susceptible in a dose-dependent manner to fluconazole and resistant to clotrimazole. Conclusions: Non-albicans Candida spp. are common agents of vulvovaginitis, and C. glabrata is the most common species in the tested patients. © 2018, Pasteur Institute of Iran. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: amphotericin B; clotrimazole; fluconazole; itraconazole, adult; antibiotic sensitivity; antifungal susceptibility; Article; Candida; Candida glabrata; Clavispora lusitaniae; clinical effectiveness; dose response; female; fungus identification; fungus isolation; gene amplification; human; Kluyveromyces marxianus; major clinical study; microbiological examination; minimum inhibitory concentration; nonhuman; nucleotide sequence; phylogeny; Pichia kudriavzevii; polymerase chain reaction; sequence analysis; vagina candidiasis
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 16 Apr 2018 05:52
Last Modified: 16 Apr 2018 05:52
URI: http://eprints.goums.ac.ir/id/eprint/9577

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