Golestan University of Medical Sciences Repository

Sensitive colorimetric assay using insulin g-quadruplex aptamer arrays on DNA nanotubes coupled with magnetic nanoparticles

Rafati, A. and Zarrabi, A. and Abediankenari, S. and Aarabi, M. and Gill, P. (2018) Sensitive colorimetric assay using insulin g-quadruplex aptamer arrays on DNA nanotubes coupled with magnetic nanoparticles. Royal Society Open Science, 5 (3).

Full text not available from this repository.

Abstract

Described here is a methodology for fabrication of a sensitive colorimetric nanoassay for measurement of insulin using G-quadruplex aptamer arrays on DNA nanotubes (DNTs) coupled with magnetic nanoparticles. The spectroscopic findings (e.g. visible spectra, velocity assay and limit of detection determination) indicated a highly sensitive performance of this new nanoassay in comparison to those results obtained from the insulin assay with non-arrayed aptamers. The clinical performance statistics (i.e. paired sample t-test, Bland–Altman plot and scatter diagram) from the newly developed assay and the enzyme-linked immunosorbent assay suggested its reliable precision and its acceptable repeatability for measurement of insulin in human sera. This is, to our knowledge, the first study for the application of magnetic nanoparticle-coupled DNTs for carrying G-quadruplex aptamers for detection of biomolecules (such as insulin) in human serum. © 2018 The Authors.

Item Type: Article
Additional Information: cited By 0
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 16 Apr 2018 05:02
Last Modified: 16 Apr 2018 05:02
URI: http://eprints.goums.ac.ir/id/eprint/9533

Actions (login required)

View Item View Item