Golestan University of Medical Sciences Repository

Association of interleukin-18 gene promoter polymorphisms with coronary artery disease in northern Iranian population

Bazgir, A. and Farazmandfar, T. and Salehi, A. and Manzari, R.S. and Attar, M. and Shahbazi, M. (2018) Association of interleukin-18 gene promoter polymorphisms with coronary artery disease in northern Iranian population. Meta Gene, 16. pp. 1-4.

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Abstract

Background: Coronary artery disease (CAD) is the leading cause of disability and mortality in the world. CAD includes a group of cardiovascular diseases such as angina, heart failure and myocardial infarction. Interleukin-18 (IL-18) is a pleiotropic proinflammatory cytokine and an inflammation inducer, which is encoded by the IL-18 gene located on chromosome 11. Different studies have shown the involvement of IL-18 in the pathogenesis of diseases such as atherosclerosis. Objective: This study investigated the association of the IL-18 gene promoter polymorphisms (− 137 and − 607) with CAD in northern Iranian population. Methods: Genomic DNA was extracted from peripheral blood of 314 patients with CAD and 364 healthy controls by a standard protocol. Sequence-Specific Primer-PCR was used for genotyping. Total RNA was extracted from peripheral blood leukocytes from 80 patients with CAD and 80 healthy individuals using Trizol reagent. Real-time PCR was used to measure the level of IL-18 mRNA in the patients and controls. Results: Analysis of genotypes revealed that the frequencies of genotype G/C and allele C in IL-18(− 137) polymorphism were significantly lower in patients group than control group (p = 0.019 and p = 0.028 respectively). No significant difference was found in the IL-18(− 607) between the patients and controls. Moreover, the level of IL-18 expression was significantly higher in CAD patients than in controls (p < 0.001). Conclusion: The results of the present study suggested a protective effect for genotype G/C of IL-18(− 137) in CAD. Moreover, due to an increased expression level of IL-18 in CAD patients, it could be considered as a prognostic and protective factor in this disease. © 2018 Elsevier B.V.

Item Type: Article
Additional Information: cited By 0
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 16 Apr 2018 04:34
Last Modified: 16 Apr 2018 04:34
URI: http://eprints.goums.ac.ir/id/eprint/9519

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