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E6-specific detection and typing of human papillomaviruses in oral cavity specimens from Iranian patients

Nikoo, H.R. and Ardebili, A. and Ravanshad, M. and Rezaei, F. and Teimoori, A. and Khanizadeh, S. and Pouriayevali, M.H. and Ajorloo, M. (2017) E6-specific detection and typing of human papillomaviruses in oral cavity specimens from Iranian patients. Iranian Biomedical Journal, 21 (6). pp. 411-416.

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Abstract

Background: Detection and quantification of human Papillomavirus (HPV) genome in oral carcinoma play an important role in diagnosis, as well as implications for progression of disease. Methods: We evaluated tissues from 50 esopharyngeal cancers collected from different regions of Iran for HPV E6 using the two type-specific primers sets. E6 gene of HPV genotypes was amplified by specific primers. The sensitivity of PCR assay was analyzed and determined using HPV-DNA-containing plasmids. Real-time PCR was utilized to determine the prevalence and HPV viral load in patients with oral cavity squamous cell carcinoma. Results: Eighteen (36) specimens were positive for HPV. Among the 18 positive specimens, 10 showed HPV-18 (55.55), and 8 specimens were positive for HPV-11 (44.44). Of the 18 infected specimens, 6 (33.32) and 12 (66.65) were identified as high-titer and low-titer viral load, respectively. Conclusions: The PCR-based assay, developed in the current study, could be used for HPV detection, quantification, and genotyping in epidemiological and clinical studies. © 2017, Pasteur Institute of Iran. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: protein E6; virus DNA, Article; cancer tissue; clinical article; controlled study; E6 gene; gene amplification; genotype; human; human cell; Human papillomavirus type 11; Human papillomavirus type 18; human tissue; Iranian people; mouth cavity; nonhuman; oropharynx squamous cell carcinoma; plasmid; prevalence; real time polymerase chain reaction; virus detection; virus load; Wart virus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 27 Dec 2017 07:50
Last Modified: 27 Dec 2017 07:50
URI: http://eprints.goums.ac.ir/id/eprint/9489

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