Golestan University of Medical Sciences Repository

Combination of interleukin-10 gene promoter polymorphisms with HLA-DRB1*15 allele is associated with multiple sclerosis

Shahbazi, M. and Abadi, J.S.A. and Roshandel, D. and Koochaki, M. and Amiri, H. and Kohansal, R. and Baghbanian, S.M. and Zamani, M. (2017) Combination of interleukin-10 gene promoter polymorphisms with HLA-DRB1*15 allele is associated with multiple sclerosis. Indian Journal of Medical Research, 145 (June). pp. 746-752.

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Abstract

Background & objectives: Multiple sclerosis (MS) is common in some ethnic groups. Interleukin-10 (IL-10) is a potent anti-inflammatory and immunosuppressive cytokine that may be an important regulator in MS disease pathogenesis. IL-10 promoter includes several single nucleotide polymorphisms and the level of IL-10 expression is related to these polymorphisms. Furthermore, loci within the histocompatibility regions are responsible for susceptibility to MS. The aim of this study was to investigate the association of IL-10 gene promoter polymorphisms and HLA-DRB1*15 allele frequencies with MS susceptibility in an Iranian population. Methods: In this study 336 MS patients and 454 healthy controls were included. Genomic DNA was purified from peripheral blood samples by a standard protocol. Genotyping was performed by the sequence-specific primer polymerase chain reaction method. Results: IL-10 −1082 G/G and IL-10 −819 C/C genotypes were more frequent in MS patients than healthy individuals. DRB1*15 allele showed a higher frequency among MS patients compared to controls. Interpretation & conclusions: The IL-10 and HLA-DRB1*15 polymorphisms were associated with the susceptibility to MS in Iranian patients. Our results suggest that gene-gene interaction of IL-10 polymorphisms and HLA-DRB1*15 alleles may be important factors in the development of MS. © 2017, Indian Council of Medical Research. All rights reserved.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: genomic DNA; HLA DR15 antigen; interleukin 10, adult; Article; blood sampling; controlled study; disease duration; DNA extraction; DNA polymorphism; ethnicity; Expanded Disability Status Scale; female; gene frequency; genetic association; genetic susceptibility; genetic variation; genome-wide association study; genotype; haplotype; human; Iranian (citizen); major clinical study; male; multiple sclerosis; nuclear magnetic resonance imaging; polymerase chain reaction; promoter region; questionnaire; single nucleotide polymorphism
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 27 Dec 2017 07:34
Last Modified: 27 Dec 2017 07:34
URI: http://eprints.goums.ac.ir/id/eprint/9486

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