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Association between cytokine gene polymorphisms and human susceptibility to brucellosis

Kabiri, F. and Rajabnia, R. and Pournajaf, A. and Gholami, M. and Bibalan, M.H. and Kassani, A. and Yaghoubi, S. and Ardebili, A. (2017) Association between cytokine gene polymorphisms and human susceptibility to brucellosis. Archives of Pediatric Infectious Diseases, 5 (3).

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Background: Allelic single nucleotide polymorphisms (SNPs) in cytokine-encoding genes can affect the degree of cytokine production, and may be related to the tendency to infectious illnesses as well as various clinical consequences. Objectives: The aim of this work was to evaluate the possible role of SNPs in the regions of the IL-10 (-592), IL-15 (-367), IL-18 (-656), IL-12 (+1188), IFN-γ (+874), TNF-α (-308), and TNF-β (+252) genes in susceptibility or resistance to brucellosis and its crucial complications. Methods: In a period of one year, 125 patients with acute brucellosis referring to 3 large public teaching hospitals were enrolled in this study. We studied the SNPs of IL-10, IL-15, IL-18, IL-12, IFN-γ, and TNF-α/β genes using the allele specific polymerase chain reaction (AS-PCR) with sequence-specific primers. Results: Frequency of GG genotype in the TNF-α and TNF-β-encoding genes increased significantly by 52% and 31.2% in patient and control groups, respectively. For IFN-γ, TA genotype was found highly enhanced in patients (60%), while the frequency of AA and TT genotypes were higher in controls (23.2% and 26.4%, respectively). The AA and CC SNPs in IL-12 were dominant in both patient (78.4%) and control (14.4%) groups. In the patient group, the GG and TT genotypes had a higher frequency for genes encoding IL-15 (33.6%) and IL-18 (89.6%). Conclusions: Based on the present study, some SNPs within the several cytokine genes, including TNF-α/β (-308/+252), IFN-γ (+874), IL-15 (-367), IL-18 (-656), and IL-12 (+1188) are related to the susceptibility or resistance to brucellosis. In order to approve the biological consequence of our results, additional investigations should be carried out in larger population groups. © 2017, Archives of Pediatric Infectious Diseases.

Item Type: Article
Additional Information: cited By 0
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 09 Oct 2017 04:56
Last Modified: 09 Oct 2017 04:56
URI: http://eprints.goums.ac.ir/id/eprint/9463

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