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Overexpression of FOXO3, MYD88, and GAPDH identified by suppression subtractive hybridization in esophageal cancer is associated with autophagy

Soltany-Rezaee-Rad, M. and Mottaghi-Dastjerdi, N. and Setayesh, N. and Roshandel, G. and Ebrahimifard, F. and Sepehrizadeh, Z. (2014) Overexpression of FOXO3, MYD88, and GAPDH identified by suppression subtractive hybridization in esophageal cancer is associated with autophagy. Gastroenterology Research and Practice, 2014.

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Abstract

To find genes involved in tumorigenesis and the development of esophageal cancer, the suppression subtractive hybridization (SSH) method was used to identify genes that are overexpressed in esophageal cancer tissues compared to normal esophageal tissues. In our SSH library, the forkhead box O3 (FOXO3), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and myeloid differentiation primary response 88 (MYD88) genes were the most highly upregulated genes, and they were selected for further studies because of their potential role in the induction of autophagy. Upregulation of these genes was also observed in clinical samples using qRT-PCR. In addition, coexpression analysis of the autophagy-related genes Beclin1, ATG12, Gabarapl, PIK3C3, and LC3 demonstrated a significant correlation between the differentially overexpressed genes and autophagy. Autophagy is an important mechanism in tumorigenesis and the development of chemoresistance in cancer cells. The upregulation of FOXO3, GAPDH, and MYD88 variants in esophageal cancer suggests a role for autophagy and provides new insight into the biology of esophageal cancer. We propose that FOXO3, GAPDH, and MYD88 are novel targets for combating autophagy in esophageal cancer. © 2014 Mohammad Soltany-Rezaee-Rad et al.

Item Type: Article
Additional Information: cited By 5
Uncontrolled Keywords: beclin 1; glyceraldehyde 3 phosphate dehydrogenase; myeloid differentiation factor 88; transcription factor FKHRL1, article; autophagy; cancer cell; cancer prognosis; cancer resistance; cancer tissue; carcinogenesis; clinical article; controlled study; esophagus cancer; gene identification; gene overexpression; human; human cell; human tissue; nucleotide sequence; protein expression; quantitative analysis; reverse transcription polymerase chain reaction; suppression subtractive hybridization; tumor gene; upregulation
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 13 Aug 2017 06:03
Last Modified: 13 Aug 2017 06:03
URI: http://eprints.goums.ac.ir/id/eprint/9389

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