Golestan University of Medical Sciences Repository

The transient receptor potential vanilloid receptor 1, TRPV1 (VR1) inhibits peristalsis in the mouse jejunum

Rahmati, R. (2012) The transient receptor potential vanilloid receptor 1, TRPV1 (VR1) inhibits peristalsis in the mouse jejunum. Archives of Iranian Medicine, 15 (7). pp. 433-438.

Full text not available from this repository.

Abstract

BACKGROUND: The transient receptor potential vanilloid receptor 1, TRPV1 previously termed the capsaicin or vanilloid receptor 1 (VR1) is a nonselective cation channel that has been cloned and is expressed predominantly in sensory neurons. TRPV1 is activated by protons as well as capsaicin. Despite extensive research, the physiological function of TRPV1 in the gastrointestinal tract and other tissues remains elusive. We have examined the effect of the selective TRPV1-receptor ligand, capsaicin, on intestinal peristalsis by studying migrating motor complexes (MMCs). METHODS: We performed experiments on Knockout mice (KO) in which the TRPV1 gene was disrupted using standard gene targeting techniques and their wildtype (WT) littermates. Jejunal contractile activity was recorded from in vitro segments of the jejunum, 4 - 5cm in length. When distended to 2 - 3 cm with H2O, the segments generated regular MMCs that were recorded as changes in intraluminal pressure. RESULTS: Capsaicin (1 - 100 nM) caused a dose-dependent inhibition of motility manifested as an increase in the interval between motor complexes (MCs) in the WT animal only, a response abolished by pre-treatment with TRPV1 antagonist capsazepine (Capz), ruthenium red (RR), and L-NAME. At higher doses of capsaicin (1 - 100 μM), periodic MCs were replaced by tonic increases in pressure upon which were superimposed continuous phasic contractions. This stimulation occurred in both KO and WT mice and was unaffected by pre-treatment with Capz, RR, and L-NAME. CONCLUSION: These data demonstrate the potential role of TRPV1 receptors in organized peristalsis in the mouse jejunum. These findings also suggest that inhibition of contractions in mouse jejunum by TRPV1-receptor activation does involve a nitric oxide synthetase (NOS) pathway.

Item Type: Article
Additional Information: cited By 5
Uncontrolled Keywords: capsaicin; capsazepine; n(g) nitroarginine; n(g) nitroarginine methyl ester; nitric oxide; ruthenium red; tetrodotoxin; vanilloid receptor 1, animal experiment; animal tissue; article; concentration (parameters); controlled study; gene function; in vitro study; inhibition kinetics; intestine contraction; intestine distension; intestine motility; intestine pressure; jejunum; male; mouse; muscle contractility; nonhuman; receptor gene; transient receptor potenial vanilloid receptor 1 gene, Animals; Capsaicin; Jejunum; Mice; Mice, Inbred C57BL; Mice, Knockout; Nitric Oxide; Nitric Oxide Synthase; Peristalsis; Transient Receptor Potential Channels; TRPV Cation Channels
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 13 Aug 2017 05:30
Last Modified: 13 Aug 2017 05:30
URI: http://eprints.goums.ac.ir/id/eprint/9380

Actions (login required)

View Item View Item