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Induction of humoral and cellular immunity against latent HSV-1 infections by DNA immunization in BALB/c mice

Ghaemi, A. and Soleimanjahi, H. and Bamdad, T. and Soudi, S. and Arefeian, E. and Hashemi, S.M. and Ebtekar, M. (2007) Induction of humoral and cellular immunity against latent HSV-1 infections by DNA immunization in BALB/c mice. Comparative Immunology, Microbiology and Infectious Diseases, 30 (4). pp. 197-210.

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Previously, we have reported that the injection of an expression vector containing Herpes simplex virus (HSV) Glycoprotein D-1 (gD-1) generated a significant antibody response in mice and protected them against HSV lethal challenge. We tested its potential to induce antibody and cell mediated immune responses in latently infected mice. Positive control group (KOS) and HSV gD-1 vaccinated mice demonstrated protection against a lethal ocularly challenge of 105.5 plaque-forming units (pfu)/eye of wild HSV-1 versus negative control groups. For neutralizing antibody titers, delayed-type hypersensitivity (DTH), lymphocyte proliferation responses, clinical evaluation and survival following lethal challenge, no considerable difference was observed between mice vaccinated with DNA plasmid and those vaccinated with KOS. KOS-vaccinated mice demonstrated the ability to completely prevent latency whereas DNA vaccinated group showed some degree of protection and displayed less latency than negative control groups and had considerably high levels of IFN-γ and strong CTL responses versus negative control groups. It can be concluded that although immunization with the DNA vaccine is more effective in both protecting mice and induction of immune response, however it could not completely block the latent infection in sensory nerves. © 2007 Elsevier Ltd. All rights reserved.

Item Type: Article
Additional Information: cited By 14
Uncontrolled Keywords: DNA vaccine; gamma interferon; herpes simplex vaccine; neutralizing antibody, animal experiment; animal model; animal tissue; antibody titer; article; Bagg albino mouse; cellular immunity; clinical evaluation; controlled study; delayed hypersensitivity; DNA immunization; female; Herpes simplex virus 1; humoral immunity; immunostimulation; infection prevention; latent virus infection; lymphocyte proliferation; mouse; nonhuman; plaque forming cell; provocation test; sensory nerve; survival rate; virus latency; wild type, Animals; Antibodies, Viral; Female; Herpes Simplex; Herpes Simplex Virus Vaccines; Herpesvirus 1, Human; Hypersensitivity, Delayed; Immunization; Interferon Type II; Lymphocyte Activation; Mice; Mice, Inbred BALB C; Neutralization Tests; Reverse Transcriptase Polymerase Chain Reaction; Trigeminal Ganglion; Vaccines, DNA; Virus Latency, Human herpesvirus 1; Mus; Simplexvirus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 13 Aug 2017 03:07
Last Modified: 13 Aug 2017 03:07
URI: http://eprints.goums.ac.ir/id/eprint/9354

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