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Gestational diabetes induced neuronal loss in CA1 and CA3 subfields of rat hippocampus in early postnatal life

Golalipour, M.J. and Kafshgiri, S.K. and Ghafari, S. (2012) Gestational diabetes induced neuronal loss in CA1 and CA3 subfields of rat hippocampus in early postnatal life. Folia Morphologica (Poland), 71 (2). pp. 71-77.

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Abstract

This study was conducted to determine the effect of gestational diabetes on the neuronal density of CA1 and CA3 subfields of the hippocampus in Wistar rat offspring. On day 1 of gestation, 10 dams were randomly allocated into two control and diabetic groups. Five animals in the diabetic group received 40 mg/kg/b.w. of streptozotocin (intraperitoneally) and the control animals were received normal saline. Six offspring of each of the gestational diabetics and controls were randomly selected in postnatal days 7 and 21. The infants were scarified and coronal sections were taken from the right dorsal hippocampus and stained with cresyl violet. The number of pyramidal cells per 10000 μm 2 area and the thickness of layers of hippocampus in CA1 and CA3 were evaluated. In postnatal day 7, the number of pyramidal neurons in CA1 significantly reduced from 118.82 ± 8.0 in the control group to 84.71 ± 3.3 neurons in gestational diabetic group, and in postnatal day 21 it significantly reduced from 112.71 ± 6.9 in the control group to 91.52 ± 8.5 in the gestational diabetic group. Also, the number of pyramidal cells of CA3 on postnatal day 7 significantly reduced from 90.33 ± 8.1 in the control group to 62.86 ± 7.2 in the gestational diabetic group, and in P21 the number of pyramidal cells significantly reduced from 78.33 ± 2.4 in the control group to 61.7 ± 9.5 cells in the diabetic group. In CA1 and CA3 the thickness of the pyramidal layer on postnatal days 7 and 21 non-significantly increased in gestational diabetics in comparison with the controls. This study showed that uncontrolled gestational diabetes reduces the pyramidal neurons of the hippocampus in rat offspring. Copyright © 2012 Via Medica.

Item Type: Article
Additional Information: cited By 12
Uncontrolled Keywords: animal; article; blood; body weight; cell count; experimental diabetes mellitus; female; glucose blood level; hippocampal CA1 region; hippocampal CA3 region; male; pathology; pregnancy; pregnancy diabetes mellitus; prenatal exposure; pyramidal nerve cell; rat; Wistar rat, Animals; Blood Glucose; Body Weight; CA1 Region, Hippocampal; CA3 Region, Hippocampal; Cell Count; Diabetes Mellitus, Experimental; Diabetes, Gestational; Female; Male; Pregnancy; Prenatal Exposure Delayed Effects; Pyramidal Cells; Rats; Rats, Wistar
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 25 Jul 2017 07:55
Last Modified: 25 Jul 2017 07:55
URI: http://eprints.goums.ac.ir/id/eprint/9331

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