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Genotyping of echinococcus granulosus isolates from human clinical samples based on sequencing of mitochondrial genes in Iran, Tehran

Nikmanesh, B. and Mirhendi, H. and Ghalavand, Z. and Alebouyeh, M. and Sharbatkhori, M. and Kia, E. and Mohebali, M. and Eghbali, M. and Rokni, M.B. (2014) Genotyping of echinococcus granulosus isolates from human clinical samples based on sequencing of mitochondrial genes in Iran, Tehran. Iranian Journal of Parasitology, 9 (1). pp. 20-27.

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Abstract

Background: The present study was aimed to investigate molecular diversity of Echinococcus granulosus isolates collected from human clinical samples using two mitochondrial genes cox1 and nad1 in Iran. Methods: Forty seven human hydatid cysts were collected through surgery from two hospitals in Tehran during 2010-2012. To determine the fertility of protoscoleces, the cyst fluids were subjected to morphological microscopic examinations. Protoscoleces were removed from each cyst and their total genomic DNAs were extracted. PCR was performed to amplify fragments of 450 and 400 base pair (bp) for cox1 and nad1 genes, respectively. Genotype diversity and sequence variation of the strains were studied by bioinformatics software and in comparison with those mtDNA sequences already deposited in GenBank. Results: Sixteen, (53.3), 13 (43.3), and 1 (3.3) samples were related to lung, liver, and spleen, respectively. The remained 17 unfertile samples were excluded from the study. From the 29 isolates, 86.7 (n=26) and 10 (n=3) were related to G1, and G3 genotypes, respectively. The sole isolate with G6 genotype was obtained from lung sample. Analysis of concatenated sequences of cox1+nad1 indicated the presence of 11 haplotypes among our strains that were related to genotypes G1 (n=9), G3 (n=1) and G6 (n=1). Conclusion: In consistent to other reports from Iran, genotypes G1, G3, and G6 were observed in our human isolates. The rate of G3 genotype was however higher than other studies implying that human can be considered as a new appropriate host for G3 genotype. Further studies with more sample size from different geographic areas of Iran are needed for E. granulosus mapping.

Item Type: Article
Additional Information: cited By 5
Uncontrolled Keywords: cyclooxygenase 1; internal transcribed spacer, article; DNA extraction; echinococcosis; Echinococcus granulosus; gene mapping; gene sequence; genetic variability; human; Iran; mitochondrial gene; nonhuman; nucleotide sequence; phylogeny; point mutation; polymerase chain reaction system; restriction fragment length polymorphism; sequence analysis
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 25 Jul 2017 07:53
Last Modified: 25 Jul 2017 07:53
URI: http://eprints.goums.ac.ir/id/eprint/9330

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