Golestan University of Medical Sciences Repository

Detection and biological characteristic of FLT3 gene mutations in children with acute leukemia

Nasiri, N. and Shaikhy, M. and Zaker, F. and Hosseini, S. and Moosavi, S.A. and Marjani, A.J. (2014) Detection and biological characteristic of FLT3 gene mutations in children with acute leukemia. Archives of Iranian Medicine, 17 (4). pp. 258-261.

Full text not available from this repository.


Introduction: FLT3 ITD and D835 mutations occur in high frequency in AML and to a lower rate in ALL patients with poor prognosis. Methods: ITD and D835 mutations were studied in 100 diagnosed acute leukemia patients including 27 AML and 73 ALL with various FAB classifications by PCR and PCR-RFLP, respectively. Subsequently, PCR products of positive samples were confirmed by sequencing analyses. Results: ITD mutations occurred in 10 of all pediatric acute leukemia, including AML and ALL. 25.9 of AML patients harbor a mutation in the ITD in various subtypes. The frequency of ITD mutations was 4 in ALL. Various insertions of nucleotides in ITD were observed, similar to those described in the literature previously. Conclusion: These preliminary data suggest that flt3-ITD mutations may play an important role in leukemogenesis in a proportion of children, particularly in the case of AML.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: vasculotropin receptor 1; CD135 antigen; FLT3 protein, human, acute granulocytic leukemia; acute leukemia; acute lymphoblastic leukemia; adolescent; allele; article; child; controlled study; female; gene duplication; gene frequency; genetic association; genetic risk; genotype; human; internal tandem duplication; leukemogenesis; male; mutation rate; polymerase chain reaction; preschool child; restriction fragment length polymorphism; school child; blood; genetics; Iran; leukocyte count; nucleotide sequence; point mutation; tandem repeat, Adolescent; Child; Child, Preschool; DNA Mutational Analysis; Female; fms-Like Tyrosine Kinase 3; Humans; Iran; Leukemia, Myeloid, Acute; Leukocyte Count; Male; Point Mutation; Precursor Cell Lymphoblastic Leukemia-Lymphoma; Tandem Repeat Sequences
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 25 Jul 2017 06:01
Last Modified: 25 Jul 2017 06:01
URI: http://eprints.goums.ac.ir/id/eprint/9313

Actions (login required)

View Item View Item