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Correlation of ciprofloxacin resistance with the AdeABC efflux system in Acinetobacter baumannii clinical isolates

Ardebili, A. and Lari, A.R. and Talebi, M. (2014) Correlation of ciprofloxacin resistance with the AdeABC efflux system in Acinetobacter baumannii clinical isolates. Annals of Laboratory Medicine, 34 (6). pp. 433-438.

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Abstract

Background: Acinetobacter baumannii is one of the most important pathogens capable of colonization in burn patients, leading to drug-resistant wound infections. This study evaluated the distribution of the AdeABC efflux system genes and their relationship to ciprofloxacin resistance in A. baumannii isolates collected from burn patients. Methods: A total of 68 A. baumannii clinical strains were isolated from patients hospitalized in Motahari Burns Center in Tehran, Iran. Ciprofloxacin susceptibility was tested by the disk diffusion and agar dilution methods. PCR amplification of the adeRS-adeB drug efflux genes was performed for all resistant and susceptible isolates. To assess the role of the drug efflux pump in ciprofloxacin susceptibility, carbonyl cyanide 3-chlorophenylhydrazone (CCCP) was used as an efflux pump inhibitor (EPI). Results: Approximately 95.6 of the Acinetobacter isolates were resistant to ciprofloxacin, with minimum inhibitory concentration (MIC) values ranging from 4 to ≥128 μg/mL. The susceptibility of 86.1 of the resistant isolates increased by factors of 2 to 64 in the presence of CCCP. All resistant isolates were positive for the adeRS-adeB genes, and 73.2 of them had mutations in the AdeRS regulatory system. Conclusions: The results showed that AdeABC genes are common in A. baumannii, which might be associated with ciprofloxacin non-susceptibility, as indicated by the observed linkage to the presence of the genes essential for the activity of the AdeABC, several single mutations occurring in the adeRS regulatory system, and an increase of ciprofloxacin susceptibility in the presence of a CCCP EPI. © The Korean Society for Laboratory Medicine

Item Type: Article
Additional Information: cited By 7
Uncontrolled Keywords: ABC transporter; antiinfective agent; bacterial DNA; bacterial protein; carbonyl 3-chlorophenylhydrazone; ciprofloxacin; hydrazone derivative, Acinetobacter baumannii; Acinetobacter Infections; antagonists and inhibitors; antibiotic resistance; chemistry; drug effects; genetics; human; isolation and purification; metabolism; microbial sensitivity test; microbiology; mutation; nucleotide sequence; polymerase chain reaction, Acinetobacter baumannii; Acinetobacter Infections; Anti-Bacterial Agents; ATP-Binding Cassette Transporters; Bacterial Proteins; Base Sequence; Ciprofloxacin; DNA, Bacterial; Drug Resistance, Bacterial; Humans; Hydrazones; Microbial Sensitivity Tests; Mutation; Polymerase Chain Reaction
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 25 Jul 2017 04:05
Last Modified: 25 Jul 2017 04:05
URI: http://eprints.goums.ac.ir/id/eprint/9310

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