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Antibody responses in human cystic hydatid disease to the laminated layer of Echinococcus granulosus

Taherkhani, H. and Zeyhle, E. and Rogan, M.T. (2007) Antibody responses in human cystic hydatid disease to the laminated layer of Echinococcus granulosus. Parasitology Research, 101 (3). pp. 647-652.

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The laminated layer of hydatid cysts of Echinococcus granulosus represents a considerable amount of parasite material. Its antigenic role, however, is unclear. Extracts of laminated layer taken from sheep cysts were analysed in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/PAGE) and were found to contain bands at 55 and 25-29 kDa, which reacted with an anti-sheep IgG antibody probe, indicating that these were likely to be host-contaminating components within the layer. However, the same bands were also recognised by a significant proportion of human hydatid patients, particularly by IgG4 antibodies, and not by negative control individuals. These individuals did not recognise immunoglobulin heavy and light chains in a sheep serum extract in the same manner. It seems likely that there are either host or parasite antigenic components at similar molecular weights or that certain parasite antigens may share epitopes with sheep immunoglobulins. The antigens at 25-29 kDa were found to be glycoproteins by lectin blot analysis and may be important markers of disease status. © 2007 Springer-Verlag.

Item Type: Article
Additional Information: cited By 13
Uncontrolled Keywords: blood extract; epitope; glycoprotein; immunoglobulin G antibody; immunoglobulin heavy chain; immunoglobulin light chain; lectin; parasite antigen, animal tissue; antibody response; article; cystic hydatid disease; echinococcosis; Echinococcus granulosus; host; human; molecular weight; nonhuman; polyacrylamide gel electrophoresis; priority journal; sheep, Animals; Antibodies, Helminth; Antigens, Helminth; Echinococcosis; Echinococcus granulosus; Electrophoresis, Polyacrylamide Gel; Glycoproteins; Humans; Immunoblotting; Sheep; Sheep Diseases, Echinococcus granulosus; Ovis aries
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Depositing User: GOUMS
Date Deposited: 22 Jul 2017 08:19
Last Modified: 22 Jul 2017 08:19
URI: http://eprints.goums.ac.ir/id/eprint/9291

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