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A plausible anti-apoptotic role of up-regulated OCT4B1 in bladder tumors

Asadzadeh, J. and Asadi, M.H. and Shakhssalim, N. and Rafiee, M.-R. and Kalhor, H.R. and Tavallaei, M. and Mowla, S.J. (2012) A plausible anti-apoptotic role of up-regulated OCT4B1 in bladder tumors. Urology Journal, 9 (3). pp. 574-580.

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Abstract

Purpose: To investigate and compare the expression of OCT4B1 between tumor and non-tumor bladder tissues. Materials and Methods: We investigated the expression of OCT4B1 in 30 tumor and non-tumor surgical specimens of the bladder, using the TaqMan real-time polymerase chain reaction approach and by carefully designing primers and probes specific for the amplification of the variant. Results: Most tumor and non-tumor samples of the bladder showed OCT4B1 expression, but its expression level was significantly higher in the tumors (P <.002). Moreover, the up-regu-lation of OCT4B1 was more significant in high-grade tumors compared to the low-grade ones (P <.05). We have also employed the RNA interference strategy to evaluate the functional role of OCT4B1 in a bladder cancer cell line, 5637. Suppression of OCT4B1 caused some changes in cell cycle distribution, and significantly elevated the rate of apoptosis in the cells. Conclusion: Our findings suggest that OCT4B1 plays a potential role in tumor initiation and/or progression of the bladder cancer. Additionally, OCT4B1 can be regarded as a new tumor marker for detection, classification, and treatment of the bladder cancer. However, more ex-perimental studies are needed to replicate our findings.

Item Type: Article
Additional Information: cited By 11
Uncontrolled Keywords: octamer transcription factor 4; tumor marker, apoptosis; article; bladder tumor; cell cycle; female; gene expression; gene expression regulation; genetics; human; male; metabolism; reverse transcription polymerase chain reaction; RNA interference; tumor cell line; upregulation, Apoptosis; Cell Cycle; Cell Line, Tumor; Female; Gene Expression; Gene Expression Regulation, Neoplastic; Humans; Male; Octamer Transcription Factor-3; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference; Tumor Markers, Biological; Up-Regulation; Urinary Bladder Neoplasms
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 22 Jul 2017 07:03
Last Modified: 22 Jul 2017 07:03
URI: http://eprints.goums.ac.ir/id/eprint/9282

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