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Rapid determination of lamivudine in human plasma by high-performance liquid chromatography

Alebouyeh, M. and Amini, H. (2015) Rapid determination of lamivudine in human plasma by high-performance liquid chromatography. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 975. pp. 40-44.

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Abstract

A simple and rapid high-performance liquid chromatographic method with spectrophotometric detection was developed for the determination of lamivudine in human plasma. Sample preparation was accomplished through protein precipitation with acetonitrile followed by aqueous phase separation using dichloromethane. Lamivudine and the internal standard acyclovir were well separated from endogenous plasma peaks on a Chromolith RP-18e column under isocratic elution with 50. mM sodium dihydrogen phosphate-triethylamine (996:4, v/v), pH 3.2 at 20 °C. Total run time at a flow-rate of 1.5. ml/min was less than 5. min. Detection was made at 278. nm. The method was specific and sensitive, with a lower quantification limit of 40. ng/ml and a detection limit of 10. ng/ml. The absolute recovery was 97.7, while the within- and between-day coefficient of variation and percent error values of the assay method were all less than 7. The linearity was assessed in the range of 40-2560 in plasma, with a correlation coefficient of greater than 0.999. The method was successfully applied to a bioequivalence study in healthy volunteers. © 2014 Elsevier B.V.

Item Type: Article
Additional Information: cited By 4
Uncontrolled Keywords: Chromatographic analysis; Dichloromethane; High performance liquid chromatography; Liquids; Phase separation; Plasma (human); Plasmas; Precipitation (chemical); Viruses, Acyclovir; Bioequivalence study; Coefficient of variation; Correlation coefficient; High-performance liquid chromatographic methods; HPLC; Lamivudine; Spectrophotometric detection, Drug products, aciclovir; dichloromethane; lamivudine; anti human immunodeficiency virus agent; lamivudine, accuracy; area under the curve; Article; drug blood level; drug determination; drug structure; high performance liquid chromatography; human; limit of detection; limit of quantitation; liquid liquid extraction; maximum plasma concentration; normal human; solid phase extraction; tablet; time to maximum plasma concentration; blood; calibration; economics; high performance liquid chromatography; precipitation; procedures; time; validation study, Anti-HIV Agents; Calibration; Chemical Precipitation; Chromatography, High Pressure Liquid; Humans; Lamivudine; Limit of Detection; Time Factors
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 22 Jul 2017 06:25
Last Modified: 22 Jul 2017 06:25
URI: http://eprints.goums.ac.ir/id/eprint/9278

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