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Effects of filgrastim on gene expression of cholinergic system in the rat hippocampus

Parhizkar, A. and Ganji, F. and Nazari, Z. and Sepehri, H. (2017) Effects of filgrastim on gene expression of cholinergic system in the rat hippocampus. National Journal of Physiology, Pharmacy and Pharmacology, 7 (5). pp. 513-516.

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Background: Filgrastim is a granulocyte colony-stimulating factor (G-CSF), which also showed effects on memory and cognation. Although recent studies have begun to explore G-CSF-related mechanisms of action in memory, little is known about its effects on cholinergic system. Since the cholinergic system has a key role in memory and some studies reported effects of G-CSF on cholinergic activity. Choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) gene expression was examined in the rat frontal cortex and hippocampus. Aims and Objectives: This study was designed to investigate the effect of G-CSF treatment on gene expression of AChE and ChAT in the frontal cortex and hippocampus during learning. Materials and Methods: In this study, male Wistar rats were treated for 2 week with filgrastim intraperitoneally at doses of 10, 50, 70 µg/kg/day. All rats received Morris water maze training (4 trials/day for 5 days). Moreover, ChAT and AChE gene expression was assessed by real-time polymerase chain reaction in the rat frontal cortex and hippocampus. Results: Over 5 days of training, filgrastim at doses 10, 50 and 70 µg/kg/day did not change the gene expression of ChAT and AChE in frontal cortex and hippocampus although at dose of 50 ug/kg a little changes but not significant observed. Conclusions: The findings observed in this study propose that G-CSF memory enhancer effects doesn’t only cholinergic and other mechanism may be involve. © 2017 Hamid Sepehri et al.

Item Type: Article
Additional Information: cited By 0
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Depositing User: GOUMS
Date Deposited: 18 Jun 2017 19:20
Last Modified: 18 Jun 2017 19:20
URI: http://eprints.goums.ac.ir/id/eprint/5125

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