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Gestational diabetes induces pancreatic beta-cells apoptosis in adult rat offspring Diabetes Gestacional Induce Apoptosis de Células Beta del Páncreas en Crías de Ratas Adultas

Nazari, Z. and Nabiuni, M. and Ghaffari, S. and Saeidi, M. and Shahriyari, A. and Golalipour, M.J. (2017) Gestational diabetes induces pancreatic beta-cells apoptosis in adult rat offspring Diabetes Gestacional Induce Apoptosis de Células Beta del Páncreas en Crías de Ratas Adultas. International Journal of Morphology, 35 (1). pp. 16-20.

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Abstract

Several studies indicated that pancreatic β-cell death occurs in both type 1 and type 2 diabetes. This experimental study was designed to determine the effect of gestational diabetes on the β-cells in 16-week-old rat offspring. By this aim, adult Wistar rats aged 10-12 weeks were randomly allocated in control and diabetic groups. The diabetic group received 40 mg/kg/body weight of streptozotocin (STZ) on day zero of gestation. After delivery, diabetic offspring of GDM mothers and controls at the age of 16 weeks were sacrificed and pancreases harvested and fixed. The number of b-cells and were counted by Gomori’s method staining. Also, apoptosis in pancreas tissue of diabetic and control offspring was detected by TUNEL assay. Results showed a significant reduction in β-cell number in offspring of GDM (p<0.05). TUNEL assay showed that the number of apoptotic cells increased in GDM compared to controls (P<0.05). This study revealed that gestational diabetes induces pancreatic beta-cells apoptosis in 16-week-old rat offspring. © 2017, Universidad de la Frontera. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 18 Jun 2017 18:36
Last Modified: 18 Jun 2017 18:36
URI: http://eprints.goums.ac.ir/id/eprint/5116

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