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The study of antioxidant enzymes and immune-related genes expression in common carp (Cyprinus carpio) fingerlings fed different prebiotics

Hoseinifar, S.H. and Ahmadi, A. and Khalili, M. and Raeisi, M. and Van Doan, H. and Caipang, C.M. (2017) The study of antioxidant enzymes and immune-related genes expression in common carp (Cyprinus carpio) fingerlings fed different prebiotics. Aquaculture Research.

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Abstract

The aim of the present study was to study the effects of different prebiotics (galacto-, fructooligosaccharide and inulin) on immune response and oxidative stress of common carp (Cyprinus carpio) fingerlings at the molecular level. A total number of 240 fish (13.85 ± 0.85 g) were supplied and randomly stocked in twelve fiberglass tanks (20 specimens per tank). Fish were fed a basal formulated diet (Control) or basal diet supplemented with equal level (2) of different prebiotics (four treatments repeated in triplicated) for 8 weeks. At the end of feeding trial, the expression of immune-related genes (interleukin 1 beta IL-1β, IL-8, IL-10, lysozyme LYZ, tumour necrosis factor alpha TNF-α and transforming growth factor beta TGF-β) were determined in head kidney and intestine tissues and the expression of antioxidant-related genes (glutathione S-transferase GST-α, glutathione reductase GR and glutathione peroxidase genes GPX) were studied in intestine. The results revealed that dietary administration of prebiotics modulated the expression of immune-related genes and the degree of expression was affected by the type of prebiotics and the organ that was used for analyses. Also, evaluation of antioxidant genes expression showed that GSTα and GR expression levels increased as a result of feeding common carp with the prebiotics. According to these findings, it can be concluded that feeding on different prebiotics had altered effects on the expression of immune and antioxidant-related genes. © 2017 John Wiley & Sons Ltd.

Item Type: Article
Additional Information: cited By 0; Article in Press
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 18 Jun 2017 18:35
Last Modified: 18 Jun 2017 18:35
URI: http://eprints.goums.ac.ir/id/eprint/5114

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