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Oscillation in expression of Adenylyl Cyclase isoforms: new insight to regulation of molecular clock

Mohamadian, S. and Golalipour, M. and Yazdani, Y. and Farazmandfar, T. and Tabarraei, A. and Shahbazi, M. (2017) Oscillation in expression of Adenylyl Cyclase isoforms: new insight to regulation of molecular clock. Biological Rhythm Research, 48 (1). pp. 113-119.

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Background: Mammalian cells contain a cell-autonomous circadian clock that couples endogenous rhythms with changes of cellular environment. Some recent reports have been shown that circadian clock regulates glucose homeostasis; but, their molecular basis is little understood. Adenylyl Cyclase (AC) is a key enzyme in glucose metabolism. We aimed to investigate circadian mRNA expression of two circadian clock genes Cry1, Cry2, and nine Adenylyl Cyclase isoforms. Methods: MCF7 cell culture shocked using horse serum. Every four hours (over a 48-h period), total RNA was extracted and cDNA was synthesized. Expression level of AC isoforms and Cry genes was evaluated by quantitative RT-PCR. Results: Two AC isoforms; AC3, AC6 showed a circadian expression pattern in which they had a significant correlation with Cry1 and Cry2 transcripts. AC3 isoform was also up-regulated after serum shock. Time lags results indicated that AC3 expression was coincided with Cry2 and leads Cry1 expression. Conclusions: Adenylyl Cyclase isoforms, especially AC3, may play an important role in molecular clock regulation and response to external factors. © 2016 Informa UK Limited, trading as Taylor & Francis Group.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Equidae; Mammalia
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Depositing User: GOUMS
Date Deposited: 18 Jun 2017 17:03
Last Modified: 18 Jun 2017 17:03
URI: http://eprints.goums.ac.ir/id/eprint/5109

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