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Expression Analysis of Previously Verified Fecal and Plasma Dow-regulated MicroRNAs (miR-4478, 1295-3p, 142-3p and 26a-5p), in FFPE Tissue Samples of CRC Patients

Ghanbari, R. and Rezasoltani, S. and Hashemi, J. and Mohamadkhani, A. and Tahmasebifar, A. and Arefian, E. and Mobarra, N. and Asadi, J. and Nazemalhosseini Mojarad, E. and Yazdani, Y. and Knuutila, S. and Malekzadeh, R. (2017) Expression Analysis of Previously Verified Fecal and Plasma Dow-regulated MicroRNAs (miR-4478, 1295-3p, 142-3p and 26a-5p), in FFPE Tissue Samples of CRC Patients. Archives of Iranian medicine, 20 (2). pp. 92-95.

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Abstract

BACKGROUND: Colorectal cancer (CRC) is one of the most common causes of cancer-related mortality worldwide. Early diagnosis of this neoplasm is critical and may reduce patients' mortality. MicroRNAs are small non-coding RNA molecules whose expression pattern can be altered in various diseases such as CRC. METHODS: In this study, we evaluated the expression levels of miR-142-3p, miR-26a-5p (their reduced expression in plasma samples of CRC patients was previously confirmed), miR-4478 and miR-1295-3p (their reduced expression in stool samples of CRC patients was previously confirmed) in tissue samples of CRC patients in comparison to healthy subjects. To achieve this purpose, total RNA including small RNA was extracted from 53 CRC and 35 normal subjects' Formalin-fixed, Paraffin-embedded (FFPE) tissue samples using the miRNeasy FFPE Mini Kit. The expression levels of these four selected miRNAs were measured using quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR). RESULTS: We found that the expression levels of miR-4478 and miR-1295b-3p (two previously down-regulated fecal miRNAs) were significantly decreased in FFPE samples of CRC patients compared to healthy controls. On the other hand, no significant differences were seen in expression levels of miR-142-3p and miR-26a-5p (two previously down-regulated circulating miRNAs) in FFPE samples between these two groups. CONCLUSION: Regarding current findings, it may be concluded that to diagnose CRC patients based on the miRNAs approach, stool samples are more likely preferable to plasma samples; nevertheless, additional studies with more samples are needed to confirm the results.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: microRNA; MIRN-4478 microRNA, human; MIRN142 microRNA, human; MIRN26A microRNA, human; tumor marker, aged; colorectal tumor; early cancer diagnosis; feces; female; gene expression regulation; genetics; human; male; middle aged; real time polymerase chain reaction, Aged; Biomarkers, Tumor; Colorectal Neoplasms; Early Detection of Cancer; Feces; Female; Gene Expression Regulation, Neoplastic; Humans; Male; MicroRNAs; Middle Aged; Real-Time Polymerase Chain Reaction
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 18 Jun 2017 16:47
Last Modified: 18 Jun 2017 16:47
URI: http://eprints.goums.ac.ir/id/eprint/5101

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