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Production and Characterization of Single Chain Nimotuzumab: An In Vitro Study

Pourafshar, M. and Safdari, Y. and Khajeniazi, S. and Yazdani, Y. and Banisadr, A. and Veisi, K. (2017) Production and Characterization of Single Chain Nimotuzumab: An In Vitro Study. International Journal of Peptide Research and Therapeutics. pp. 1-8.

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Abstract

Recombinant expression and EGFR-binding activity assay of single chain nimotuzumab (nimotuzumab scFv) is reported in this study. The scFv was produced in VH-linker-VL format in Origami™ 2(DE3)pLysS bacterial cells and purified using Ni-TNA resin column. 3-D structure prediction using I-TASSER (Iterative Threading Assembly Refinement) server and analyzing the predicted models using YASARA (Yet Another Scientific Artificial Reality Application) viewer revealed that VH and VL domains assemble into a correctly-folded single chain antibody that is able to bind EGFR. The scFv was evaluated in ELISA and western blot tests and proven to be able to bind EGFR-overexpressing cancer cells (A-431 cells in an efficient manner but unable to recognize cancer cells expressing low levels of EGFR (MCF-7 breast cancer cells). © 2017 Springer Science+Business Media New York

Item Type: Article
Additional Information: cited By 0; Article in Press
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 18 Jun 2017 15:06
Last Modified: 18 Jun 2017 15:06
URI: http://eprints.goums.ac.ir/id/eprint/5060

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