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Tetanus neurotoxin HCC protein commits T cell to IFN-γ producing cells

Torabi Goudarzi, S. and Hajivalili, M. and Hosseini, M. and Ghafari Khamene, M. and Yazdani, Y. and Sadreddini, S. and Miahipour, A. and Younesi, V. and Yousefi, M. (2016) Tetanus neurotoxin HCC protein commits T cell to IFN-γ producing cells. Cellular and Molecular Biology, 62 (3). pp. 20-24.

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A protective response against tetanus toxin and toxoid demands efficient specific T cell and B cell responses. Tetanus neurotoxin (TeNT), a 150 kDa polypeptide, is the main cause of tetanus disease. TeNT consists of two structurally distinct chains, a 50 kDa N-terminal light (L) and a 100 kDa C-terminal heavy (H) chain. C-terminal heavy (H) chain (fragment C) has two sub-domains named as proximal HCN and carboxy sub-domain or HCC. Beside neural binding property, HCC has been recently found as an immunodominant module of TeNT. In the present study, we investigated the effects of recombinant HCC (rHCC) on the expression of lineage specific transcription factors and secretion of a panel of functional cytokines including IFN-γ, IL-4, and IL-17 from purified human T cells. Our results revealed that T-bet transcript level, as TH1 specific transcription factor, was significantly increased in the cells treated with 10 and 20 μg/ml of rHCC following 48 h treatment(p < 0.05). Treated purified human T cells with rHCC showed significant increase in IFN-γ mRNA level and cytokine secretion, but not IL-4 and IL-17, following 48 h treatment. In conclusion, our results showed that treatment of T cells with r HCC resulted in development of Th1 lineage phenotype, which might lead to a specific and protective antibody mediated response against tetanus toxin. © 2016 by the C.M.B. Association. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Depositing User: GOUMS
Date Deposited: 13 Jun 2017 05:38
Last Modified: 13 Jun 2017 07:43
URI: http://eprints.goums.ac.ir/id/eprint/4926

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