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Staphylococcal cassette chromosome mec types among methicillin-resistant staphylococcus aureus in northern Iran

Taherirad, A. and Jahanbakhsh, R. and Shakeri, F. and Anvary, S. and Ghaemi, E.A. (2016) Staphylococcal cassette chromosome mec types among methicillin-resistant staphylococcus aureus in northern Iran. Jundishapur Journal of Microbiology, 9 (8).

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Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a common cause of nosocomial and community-acquired infections around the world. Staphylococcal cassette chromosome mec (SCCmec) typing methods are often used to study MRSA molecular epidemiology. Objectives: The current study was designed to explore the distribution profiles of different SCCmec types among methicillinresistant S. aureus strains isolated from hospitals in Gorgan, in northern Iran, and to correlate the types into observed bacterial virulence factors. Materials and Methods: Staphylococcal cassette chromosome mec typing of 62 MRSA strains isolated from patients and healthcare workers in Gorgan was performed using multiplex polymerase chain reaction (PCR) assay. The prevalence of the strains was then compared according to isolation source, antibiotic susceptibility profiles, biofilm production, and the presence of the Panton- Valentine gene in isolates. Results: The mostcommon SCCmec type was type III, with a frequency rate of 76, followed by types IV, I, and V, with frequency rates of 11.2, 4.8, and 3.2, respectively; three isolates (4.8) were not typeable by this method. SCCmec type I was only isolated from blood culture, and types IV and V were mainly isolated from wounds and urine samples; SCCmec type III was isolated from all of the clinically samples. All of the MRSA strains that were isolated from healthy carriers were type III. Multidrug resistance in the type III strains was higher compared to the other types. The frequencies of Panton-Valentine and biofilm production were significantly lower in the type III strains compared to the other SCCmec types (P < 0.05). Conclusions: Similarly to other geographical regions of Iran, the SCCmec type III MRSA strain was the most frequently isolated strain from patients in Gorgan. Staphylococcal cassette chromosomemectype III showed fewer virulence factors compared to other SCCmec types. © 2016. Ahvaz Jundishapur University of Medical Sciences.

Item Type: Article
Additional Information: cited By 1
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 13 Jun 2017 05:18
Last Modified: 13 Jun 2017 05:18
URI: http://eprints.goums.ac.ir/id/eprint/4917

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