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Involvement of the multidrug efflux pumps in betalactams resistant Pseudomonas aerugionsa clinical isolates collected from burn patients in Iran

Bijari, A. and Azimi, L. and Fallah, F. and Ardebili, A. and Lari, E.R. and Lari, A.R. (2016) Involvement of the multidrug efflux pumps in betalactams resistant Pseudomonas aerugionsa clinical isolates collected from burn patients in Iran. Infectious Disorders - Drug Targets, 16 (3). pp. 172-177.

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Abstract

Background: Multi-drug resistant P. aeruginosa has been increasing worldwide. Various mechanisms create this cross resistance in this bacterium including, acquisition beta-lactamases and intrinsic mechanisms, such as the presence of multidrug efflux pumps. Generally, multidrug efflux pumps inhibitor is used as a phenotypic method for the detection of active efflux pump systems. Methods: In this study, 100 P. aeruginosa were collected from burn patients. Cefepime and imipenem resistant strains were isolated by disc diffusion agar and MIC. Carbonyl cyanide m-chlorophenyl hydrazone (CCCP), H+ conductor was used to detect activities of multidrug efflux pumps. The multidrug efflux genes of mexA, mexC and mexX were detected by PCR. Results: 87 and 79 of isolates were cefepime and imipenem resistant respectively. Only 18 of cefepime and imipenem resistant strains were inhibited by CCCP. 98 and 97 of isolates harbored mexA and mexC genes, respectively, while all of them harbored mexX. Conclusion: Thus, cross resistance in P. aeruginosa isolates can be obtained by multidrug efflux pump systems. Therefore, the inhibition of multidrug efflux pumps can be very helpful for the treatment of infections caused by multi drug resistant P. aeruginosa. © 2016 Bentham Science Publishers.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: beta lactamase; carbonyl cyanide chlorophenylhydrazone; carrier protein; cefepime; cephalosporin derivative; imipenem; MexA protein, Pseudomonas aeruginosa; MexC protein, Pseudomonas aeruginosa; outer membrane protein, antibiotic resistance; burn; drug effects; gene; genetics; human; Iran; isolation and purification; metabolism; microbial sensitivity test; microbiology; multidrug resistance; phenotype; polymerase chain reaction; Pseudomonas aeruginosa; Pseudomonas infection, Bacterial Outer Membrane Proteins; beta-Lactam Resistance; beta-Lactamases; Burns; Carbonyl Cyanide m-Chlorophenyl Hydrazone; Cephalosporins; Drug Resistance, Multiple, Bacterial; Genes, MDR; Humans; Imipenem; Iran; Membrane Transport Proteins; Microbial Sensitivity Tests; Phenotype; Polymerase Chain Reaction; Pseudomonas aeruginosa; Pseudomonas Infections
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: UNSPECIFIED
Depositing User: GOUMS
Date Deposited: 12 Jun 2017 09:45
Last Modified: 12 Jun 2017 09:45
URI: http://eprints.goums.ac.ir/id/eprint/4898

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