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Sequence variation in mitochondrial cox1 and nad1 genes of ascaridoid nematodes in cats and dogs from Iran

Mikaeili, F. and Mirhendi, H. and Mohebali, M. and Hosseini, M. and Sharbatkhori, M. and Zarei, Z. and Kia, E.B. (2015) Sequence variation in mitochondrial cox1 and nad1 genes of ascaridoid nematodes in cats and dogs from Iran. Journal of Helminthology, 89 (4). pp. 496-501.

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Abstract

The study was conducted to determine the sequence variation in two mitochondrial genes, namely cytochrome c oxidase 1 (pcox1) and NADH dehydrogenase 1 (pnad1) within and among isolates of Toxocara cati, Toxocara canis and Toxascaris leonina. Genomic DNA was extracted from 32 isolates of T. cati, 9 isolates of T. canis and 19 isolates of T. leonina collected from cats and dogs in different geographical areas of Iran. Mitochondrial genes were amplified by polymerase chain reaction (PCR) and sequenced. Sequence data were aligned using the BioEdit software and compared with published sequences in GenBank. Phylogenetic analysis was performed using Bayesian inference and maximum likelihood methods. Based on pairwise comparison, intra-species genetic diversity within Iranian isolates of T. cati, T. canis and T. leonina amounted to 0-2.3, 0-1.3 and 0-1.0 for pcox1 and 0-2.0, 0-1.7 and 0-2.6 for pnad1, respectively. Inter-species sequence variation among the three ascaridoid nematodes was significantly higher, being 9.5-16.6 for pcox1 and 11.9-26.7 for pnad1. Sequence and phylogenetic analysis of the pcox1 and pnad1 genes indicated that there is significant genetic diversity within and among isolates of T. cati, T. canis and T. leonina from different areas of Iran, and these genes can be used for studying genetic variation of ascaridoid nematodes. © Cambridge University Press 2014.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: cytochrome c oxidase; cytochrome c oxidase 1; genomic DNA; reduced nicotinamide adenine dinucleotide dehydrogenase; reduced nicotinamide adenine dinucleotide dehydrogenase 1; unclassified drug, amplicon; Article; Australia; Canis lupus; cat; China; computer program; controlled study; DNA extraction; DNA sequence; dog; GenBank; genetic variability; geographic distribution; haplotype; Iran; Malaysia; maximum likelihood method; mitochondrial gene; mitochondrial genome; nonhuman; nucleotide sequence; parasite isolation; phylogeny; polymerase chain reaction; sequence analysis; single strand conformation polymorphism; Toxascaris leonina; Toxocara canis; Toxocara cati, Ascaridoidea; Canis familiaris; Nematoda; Toxascaris leonina; Toxocara canis; Toxocara cati
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Sep 2016 09:14
Last Modified: 16 Jan 2017 10:37
URI: http://eprints.goums.ac.ir/id/eprint/4824

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