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Cytoprotective effects of melatonin against amitriptyline-induced toxicity in isolated rat hepatocytes

Taziki, S. and Sattari, M.R. and Dastmalchi, S. and Eghbal, M.A. (2015) Cytoprotective effects of melatonin against amitriptyline-induced toxicity in isolated rat hepatocytes. Advanced Pharmaceutical Bulletin, 5 (3). pp. 329-334.

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Purpose: Amitriptyline, one of the commonly used tricyclic antidepressants, caused rare but severe hepatotoxicity in patients who received it continuously. Previous findings showed that the intermediate metabolites of amitriptyline produced by CYP450 are involved in hepatic injury. Melatonin is an antiaging and antioxidant hormone synthesized from pineal gland. The aim of present study was to evaluate the protective role of melatonin in an in vitro model of isolated rat hepatocytes. Methods: Markers such as cell viability, reactive oxygen species formation, lipid peroxidation, mitochondrial membrane potential, and hepatocytes glutathione content were evaluated every 60 minutes for 180 minutes. Results: Present results indicated that administration of 1mM of melatonin effectively reduced the cell death, ROS formation and lipid peroxidation, mitochondrial membrane potential collapse, and reduced cellular glutathione content caused by amitriptyline. Conclusion: Our results indicated that melatonin is an effective antioxidant in preventing amitriptyline-induced hepatotoxicity. We recommend further in vivo animal and clinical trial studies on the hepatoprotective effects of melatonin in patients receiving amitriptyline. © 2015 The Authors.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: amitriptyline; glutathione; melatonin, animal cell; animal tissue; antioxidant activity; Article; cell protection; cell viability; controlled study; cytotoxicity; drug effect; drug mechanism; drug response; in vitro study; lipid peroxidation; liver cell; male; mitochondrial membrane potential; nonhuman; oxidative stress; rat
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Sep 2016 09:08
Last Modified: 24 Dec 2016 09:07
URI: http://eprints.goums.ac.ir/id/eprint/4820

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