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Gold nanoparticle and mean inactivation dose of human intestinal colon cancer HT-29 cells

Arab-Bafrani, Z. and Saberi, A. and Tahmasebi Birgani, M.J. and Shahbazi-Gahrouei, D. and Abbasian, M. and Fesharaki, M. (2015) Gold nanoparticle and mean inactivation dose of human intestinal colon cancer HT-29 cells. Jundishapur Journal of Natural Pharmaceutical Products, 10 (4).

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Background: Mean inactivation dose is a useful radiobiological parameter for the comparison of human cell survival curves. Objectives: Given the importance and accuracy of these parameters, in the present study, the radio sensitivity enhancement of colon cancer (HT-29) cells in the presence of gold nanoparticles (GNPs) were studied using the mean inactivation dose (MID). Materials and Methods: Naked-GNPs with 50 nm diameters were incubated with HT-29 cells. The cytotoxicity and uptake of these particles on HT-29 cells were assessed. After determining the optimum GNPs concentration, the cells were incubated with gold nanoparticle for 24 hours. The change in the MID value as well as the radio sensitization enhancement under irradiation with 9 MV X-ray beams in the presence of GNPs were evaluated by multiple (3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)MTS assay. Results: Cell survival in the presence of GNPs was more than 90% and the maximum uptake of GNPs was observed at 60 μM of gold nanoparticles. In contrast, in the presence of GNPs combined with radiation, cell survival and MID value significantly decreased, so that the radio sensitization enhancement was 1.4. Conclusions: Due to the significant reduction in the mean inactivation dose of colon cancer cells in the presence of gold nanoparticles, it seems that GNPs are suitable options to achieve a new approach in order to improve radiotherapy efficiency without increasing the prescribed radiation dose.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: gold nanoparticle, Article; atomic absorption spectrometry; cancer cell culture; cancer radiotherapy; cell survival; cell viability; colorectal cancer; cytotoxicity; dosimetry; human; human cell; in vitro study; MTS assay; radiation dose; radiosensitization
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Sep 2016 09:07
Last Modified: 09 Jan 2017 07:44
URI: http://eprints.goums.ac.ir/id/eprint/4819

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