Golestan University of Medical Sciences Repository

Prevalence and distribution of BK virus subtypes in renal transplant recipients referred to Golestan hospital in Ahvaz, Iran

Kaydani, G.A. and Makvandi, M. and Samarbafzadeh, A. and Shahbazian, H. and Fard, M.H. (2015) Prevalence and distribution of BK virus subtypes in renal transplant recipients referred to Golestan hospital in Ahvaz, Iran. Jundishapur Journal of Microbiology, 8 (3).

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Abstract

Background: BK virus (BKV) belongs to the human Polyomaviridae and the primary BKV infection is occurred during childhood then the virus could be latent through life, especially in the kidneys and urinary system. It became reactive after an immunocompromised status, such as pregnancy or transplantation. Isolated BKV from different locations of the world is grouped into four subtypes using serological and genotyping methods. The BKV subtype I is the dominant one and has worldwide distribution. Objectives: According to our knowledge, there are no data about the BKV prevalence and its genotypes in southwest part of Iran. Considering the high prevalence of renal failure and kidney transplant patients in this part, and the role of BKV in graft rejection, this study aimed to determine the prevalence of BKV infection in renal transplant recipients referred to Golestan Hospital in Ahvaz City, Iran. Patients and Methods: Urine samples were collected from 122 kidney transplant recipients referred to Golestan Hospital in Ahvaz, southwest of Iran. The extracted DNA was amplified by Polymerase Chain Reaction, and subtype of each positive sample was determined using Restriction Fragment Length Polymorphism (RFLP) and sequencing methods. Results: From all study population, 51/122 (41.8%) urine samples were positive for BKV DNA and the other samples were negative (71/122). Forty-eight cases (94.11%) were subtype I and 3 others (5.89%) were subtype IV using the RFLP method. None of the patient’s urine samples were positive for subtypes II and III. Conclusions: Our work is the second study in Iran and considering huge numbers of transplantation in Iran and Khuzestan Province, south western of Iran, in addition to the role of this virus in kidney transplant rejection, routine evaluation of BKV positivity is recommended both for graft recipient and donors. This helps better transplantation result and may prevent graft rejection. © 2015, Ahvaz Jundishapur University of Medical Sciences.

Item Type: Article
Additional Information: cited By 3
Uncontrolled Keywords: Article; BK virus; bk virus infection; controlled study; DNA extraction; female; genotype; genotyping technique; graft recipient; human; Iran; kidney failure; kidney graft rejection; kidney transplantation; major clinical study; male; patient referral; polymerase chain reaction; prevalence; questionnaire; restriction fragment length polymorphism; serology, BK polyomavirus; Polyomaviridae
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Sep 2016 08:55
Last Modified: 10 Sep 2016 08:55
URI: http://eprints.goums.ac.ir/id/eprint/4813

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