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Chitosan nanoparticles as a potential nonviral gene delivery for HPV-16 E7 into mammalian cells

Tahamtan, A. and Tabarraei, A. and Moradi, A. and Dinarvand, M. and Kelishadi, M. and Ghaemi, A. and Atyabi, F. (2015) Chitosan nanoparticles as a potential nonviral gene delivery for HPV-16 E7 into mammalian cells. Artificial Cells, Nanomedicine and Biotechnology, 43 (6). pp. 366-372.

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Abstract

Chitosan nanoparticles (CS NPs) were prepared as a carrier for Human papillomavirus type 16 HPV-16) E7 gene and their gene transfection ability were evaluated in vitro . The plasmid expressing green fl uorescent protein (pEGFP) was used as a reporter gene. Gel electrophoresis demonstrated full binding of CS NPs with the pDNA. The transfection of CS-pEGFP NPs was effi cient in CHO cells and the expression of green fl uorescent proteins was well observed. The expression of E7 proteins was confi rmed under SDS-PAGE and western blot analysis. As a conclusion CS NPs may serve as an eff ective nonviral carrier for delivery of nucleotides into eukaryotic cells. Copyright © 2014 Informa Healthcare USA, Inc.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Cell culture; Cells; Chitin; Chitosan; Cytology; Electrophoresis; Gene transfer; Genes; Mammals; Nanoparticles; Proteins, Chitosan nanoparticles; CHO cell; Gel electrophoresis; Gene Delivery; HPV-16 E7; Human papillomavirus; Non-viral gene delivery; Western-blot analysis, Molecular biology, chitosan nanoparticle; green fluorescent protein; protein E7; chitosan; enhanced green fluorescent protein; nanoparticle; oncogene protein E7, Human papillomavirus type 16; protein E7, animal cell; Article; cell viability; controlled study; cytotoxicity; fluorescence microscopy; gel electrophoresis; in vitro gene transfer; in vitro study; mammal cell; nonhuman; nonviral gene delivery system; polyacrylamide gel electrophoresis; protein expression; Western blotting; animal; chemistry; CHO cell line; Cricetulus; gene expression; gene transfer; genetics; Human papillomavirus type 16; metabolism; plasmid; reporter gene, Animals; Blotting, Western; Chitosan; CHO Cells; Cricetulus; Electrophoresis, Polyacrylamide Gel; Gene Expression; Gene Transfer Techniques; Genes, Reporter; Green Fluorescent Proteins; Human papillomavirus 16; Nanoparticles; Papillomavirus E7 Proteins; Plasmids
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Sep 2016 08:11
Last Modified: 15 Jan 2017 07:03
URI: http://eprints.goums.ac.ir/id/eprint/4788

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