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Decreased expression of fecal miR-4478 and miR-1295b-3p in early-stage colorectal cancer

Ghanbari, R. and Mosakhani, N. and Asadi, J. and Nouraee, N. and Mowla, S.J. and Poustchi, H. and Malekzadeh, R. and Knuutila, S. (2015) Decreased expression of fecal miR-4478 and miR-1295b-3p in early-stage colorectal cancer. Cancer Biomarkers, 15 (2). pp. 195-201.

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Abstract

BACKGROUND: Colorectal cancer (CRC) is a major cause of cancer-related deaths world-wide. Detection of molecular markers in stool samples is a promising strategy for CRC screening. MicroRNAs (miRNAs) are short, non-coding RNA molecules that are commonly dysregulated in neoplasia. OBJECTIVE: The objective of this study was to evaluate the fecal miRNAs differentiation between early-stage CRC patients and healthy subjects. METHODS: Stool samples were collected from 40 patients with early stage (I, II) CRC and 16 healthy controls. RNA was extracted from all samples using miRNAeasy Mini Kits. MiRNA microarray expression profiling was performed with Agilent's miRNA Microarray system on 12 CRC and 8 normal stool samples. The expression levels of miR-4478 and miR-1295b-3p were determined by the SYBR Green miScript PCR system. RESULTS: In profiling study, we found 215 down-regulated miRNAs in CRC group. Furthermore, in validation study we found that the expression levels of fecal miR-4487 and miR-1295b-3p were significantly decreased in CRC patients compared to healthy controls. CONCLUSIONS: The expression of miR-4478 and miR-1295b-3p were significantly diminished in stool samples of CRC patients with early stage (I, II) in comparison with normal group. These miRNAs maybe use as potential non-invasive molecular markers for CRC diagnosis, but further studies are needed. © 2015 - IOS Press and the authors. All rights reserved.

Item Type: Article
Additional Information: cited By 5
Uncontrolled Keywords: microRNA; microRNA 1295b 3p; microRNA 4478; unclassified drug, adult; Article; cancer patient; cancer staging; clinical article; colorectal cancer; controlled study; feces analysis; female; human; male; microarray analysis; priority journal; protein expression; RNA extraction; RNA isolation; validation study
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Sep 2016 08:09
Last Modified: 24 Dec 2016 09:13
URI: http://eprints.goums.ac.ir/id/eprint/4787

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