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Association between CTLA-4 gene polymorphism and the risk of systemic lupus erythematosus: Brief report

Shojaa, M. and Aghaie, M. and Amoli, M. and Khashayar, P. and Javid, N. and Shakeri, F. and Qorbani, M. and Mohebbi, R. (2015) Association between CTLA-4 gene polymorphism and the risk of systemic lupus erythematosus: Brief report. Tehran University Medical Journal, 73 (2). pp. 127-131.

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Abstract

Background: Cytotoxic lymphocyte antigen-4 (CTLA-4) plays an important role in regulating T cell activation. CTLA-4 gene polymorphisms are related with genetic susceptibility to various autoimmune diseases, including systemic lupus erythematosus (SLE). We analyzed the role of CTLA-4 polymorphisms at positions -318CT in patients who suffer from SLE. Methods: This study was performed on 180 SLE patients referred to 5th Azar University Hospital in Gorgan, Iran. Three hundred and four ethnically-and age-matched healthy controls with no history of autoimmune diseases entered the study between 5th May 2008 and 23rd October 2009. DNA was extracted from blood samples according to the standard procedure. Polymerase chain reaction- restriction fragments length polymorphism (PCR-RFLP) was used to analyze the genotype and allele frequencies of this polymorphism. PCR was carried out using the following primers: forward 5′- AAATGAATTGGACTGGATGGT-3′ and reverse 5′-TTACGAGAAAGGAAGCCGT G-3′. The frequency of alleles and genotypes were assessed using direct counting. Chisquare test and Fisher’s exact test were used to compare the association between the alleles and genotype frequencies and SLE. P<0.05 were considered statistically significant. Results: The CC genotype was observed in 94.5% of the SLE patients and 82.4% of the controls; the difference was statistically significant (P=0.0001, OR=3.51, CI95%=1.77- 7.53). The CT genotype, on the other hand, was more frequently observed in the control group (17.1% vs. 5.5%, P=0.0001, OR=0.28). T allele was significantly more common in the controls compared to SLE patients (P=0.0001, OR=0.26, CI95%=0.13-0.53). Conclusion: Our results suggest that the -318C/T polymorphism of CTLA-4 gene might play a significant role in the genetic susceptibility to SLE. Therefore, further studies on populations, especially from other Middle East countries, are needed to confirm our results. © 2015, Tehran University of Medical Sciences. All Rights Reserved.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Article; controlled study; ctla4 gene; DNA polymorphism; gene; gene frequency; genotype; human; major clinical study; polymerase chain reaction; restriction fragment length polymorphism; systemic lupus erythematosus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Sep 2016 07:26
Last Modified: 14 Jan 2017 10:30
URI: http://eprints.goums.ac.ir/id/eprint/4779

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