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Preliminary assessment of various additives on the specific reactivity of anti- rHBsAg monoclonal antibodies

Yazdani, Y. and Mohammadi, S. and Yousefi, M. and Shokri, F. (2015) Preliminary assessment of various additives on the specific reactivity of anti- rHBsAg monoclonal antibodies. Avicenna Journal of Medical Biotechnology, 7 (4). pp. 145-150.

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Abstract

Background: Antibodies have a wide application in diagnosis and treatment. In order to maintain optimal stability of various functional parts of antibodies such as antigen binding sites, several approaches have been suggested. Using additives such as polysaccharides and polyols is one of the main methods in protecting antibodies against aggregation or degradation in the formulation. The aim of this study was to evaluate the protective effect of various additives on the specific reactivity of monoclonal antibodies (mAbs) against recombinant HBsAg (rHBsAg) epitopes. Methods: To estimate the protective effect of different additives on the stability of antibody against conformational epitopes (S3 antibody) and linear epitopes (S7 and S11 antibodies) of rHBsAg, heat shock at 37°C was performed in liquid and solid phases. Environmental factors were considered to be constant. The specific reactivity of antibodies was evaluated using ELISA method. The data were analyzed using SPSS software by Mann-Whitney nonparametric test with the confidence interval of 95%. Results: Our results showed that 0.25 M sucrose, 0.04 M trehalose and 0.5% BSA had the most protective effect on maintaining the reactivity of mAbs (S3) against conformational epitopes of rHBsAg. Results obtained from S7 and S11 mAbs against linear characteristics showed minor differences. The most efficient protective additives were 0.04 M trehalose and 1 M sucrose. Conclusion: Nowadays, application of appropriate additives is important for increasing the stability of antibodies. It was concluded that sucrose, trehalose and BSA have considerable effects on the specific reactivity of anti rHBsAg mAbs during long storage. © 2015, Avicenna Journal of Medical Biotechnology. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: bovine serum albumin; drug additive; epitope; glycerol; hepatitis B surface antigen; monoclonal antibody; recombinant protein; sorbitol; sucrose; trehalose, antigen antibody reaction; antigen binding; Article; cross reaction; enzyme immunoassay; enzyme linked immunosorbent assay; immunoreactivity; protein stability; serotype; storage
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Sep 2016 07:20
Last Modified: 14 Jan 2017 05:11
URI: http://eprints.goums.ac.ir/id/eprint/4773

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