Golestan University of Medical Sciences Repository

Human adenoviruses role in ophthalmic pterygium formation

Kelishadi, M. and Kelishadi, M. and Moradi, A. and Javid, N. and Bazouri, M. and Tabarraei, A. (2015) Human adenoviruses role in ophthalmic pterygium formation. Jundishapur Journal of Microbiology, 8 (4).

PDF - Published Version
Download (138kB) | Preview


Background: Ophthalmic pterygium is a common benign lesion of unknown origin and the pathogenesis might be vision-threatening. This problem is often associated with exposure to solar light. Recent evidence suggests that potentially oncogenic viruses such as human papillomavirus and Epstein-Barr virus may be involved in the pathogenesis of pterygia. Expression of specific adenovirus genes such as E1A and E1B, which potentially have many functions, may contribute to their oncogenic activity as well as relevance to cellular immortalization. Objectives: For the first time, we aimed to investigate involvement of adenoviruses in pterygium formation. Patients and Methods: Fifty tissue specimens of pterygium from patients undergoing pterygium surgery (as cases), 50 conjunctival swab samples from the same patients and 10 conjunctival biopsy specimens from individuals without pterygium such as patients undergoing cataract surgery (as controls) were analyzed for evidence of adenovirus infection with polymerase chain reaction using specific primers chosen from the moderately conserved region of the hexon gene. Furthermore, ß-globin primers were used to access the quality of extracted DNA. Data was analyzed using SPSS (version 16) software. Results: Of 50 patients, 20 were men and 30 women with mean age of 61.1 ± 16.9 years ranged between 22 and 85 years. All samples of pterygia had positive results for adenoviruses DNA with polymerase chain reaction, but none of the negative control groups displayed adenoviruses. The pterygium group and the control groups were ß-globin positive. Direct sequencing of PCR products confirmed Adenovirus infection. Conclusions: Adenoviruses might act as a possible cause of pterygium formation and other factors could play a synergistic role in the development. However, further larger studies are required to confirm this hypothesis. © 2015, Kowsar Medical Publishing Company. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: hemoglobin beta chain, Adenoviridae; adenovirus infection; adult; aged; Article; biopsy technique; clinical article; conjunctiva; controlled study; cross-sectional study; disease course; disease severity; DNA extraction; E1A gene; E1B gene; female; gene expression; human; human tissue; Iran; male; nonhuman; pathogenesis; polymerase chain reaction; prevalence; promoter region; pterygium; surgical swab; very elderly; virus gene; virus identification, Adenoviridae; Human adenovirus; Human herpesvirus 4; Human papillomavirus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Sep 2016 07:18
Last Modified: 16 Jan 2017 04:44
URI: http://eprints.goums.ac.ir/id/eprint/4771

Actions (login required)

View Item View Item