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Genetic variability of Taenia saginata inferred from mitochondrial DNA sequences

Rostami, S. and Salavati, R. and Beech, R.N. and Babaei, Z. and Sharbatkhori, M. and Harandi, M.F. (2015) Genetic variability of Taenia saginata inferred from mitochondrial DNA sequences. Parasitology Research, 114 (4). pp. 1365-1376.

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Abstract

Taenia saginata is an important tapeworm, infecting humans in many parts of the world. The present study was undertaken to identify inter- and intraspecific variation of T. saginata isolated from cattle in different parts of Iran using two mitochondrial CO1 and 12S rRNA genes. Up to 105 bovine specimens of T. saginata were collected from 20 slaughterhouses in three provinces of Iran. DNA were extracted from the metacestode Cysticercus bovis. After PCR amplification, sequencing of CO1 and 12S rRNA genes were carried out and two phylogenetic analyses of the sequence data were generated by Bayesian inference on CO1 and 12S rRNA sequences. Sequence analyses of CO1 and 12S rRNA genes showed 11 and 29 representative profiles respectively. The level of pairwise nucleotide variation between individual haplotypes of CO1 gene was 0.3–2.4 % while the overall nucleotide variation among all 11 haplotypes was 4.6 %. For 12S rRNA sequence data, level of pairwise nucleotide variation was 0.2–2.5 % and the overall nucleotide variation was determined as 5.8 % among 29 haplotypes of 12S rRNA gene. Considerable genetic diversity was found in both mitochondrial genes particularly in 12S rRNA gene. © 2015, Springer-Verlag Berlin Heidelberg.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: cytochrome c oxidase; cytochrome c oxidase subunit 1; genomic DNA; helminth DNA; mitochondrial DNA; nucleotide; RNA 12S; unclassified drug; helminth protein; mitochondrial DNA; ribosome RNA; RNA, ribosomal, 12S, Article; bovine; controlled study; DNA extraction; DNA sequence; gene sequence; genetic variability; haplotype; intraspecific variation; Iran; mitochondrial gene; nonhuman; parasite isolation; phylogeny; polymerase chain reaction; priority journal; sequence analysis; slaughterhouse; Taenia saginata; animal; cattle disease; classification; genetics; human; isolation and purification; molecular genetics; parasitology; Taenia saginata; taeniasis; veterinary, Animals; Cattle; Cattle Diseases; DNA, Helminth; DNA, Mitochondrial; Electron Transport Complex IV; Genetic Variation; Haplotypes; Helminth Proteins; Humans; Iran; Molecular Sequence Data; Phylogeny; Polymerase Chain Reaction; RNA, Ribosomal; Taenia saginata; Taeniasis
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Sep 2016 07:05
Last Modified: 15 Jan 2017 18:51
URI: http://eprints.goums.ac.ir/id/eprint/4763

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