Golestan University of Medical Sciences Repository

The simultaneous effect of valproic acid and gamma radiation on telomerase activity and bax and Bcl-2 protein levels in MCF-7 breast cancer cell line

Binabaj, M.M. and Hosseini, S.A. and Khoshnazar, A.K. and Asadi, J. (2015) The simultaneous effect of valproic acid and gamma radiation on telomerase activity and bax and Bcl-2 protein levels in MCF-7 breast cancer cell line. Jundishapur Journal of Natural Pharmaceutical Products, 10 (3).

[img]
Preview
PDF - Published Version
Download (809kB) | Preview

Abstract

Background: Breast cancer is one of the most prevalent types of cancer. Factors such as ionizing radiation and chemotherapeutic agents can trigger apoptosis and cancer cell death. An anticonvulsant drug named Valproic acid is a histone deacetylase inhibitor that shows promising anti-tumor effects in a variety of cancers. Telomerase is a ribonucleoprotein enzyme that activated in cancer cells and lead to telomeres shortening inhibition and triggering the apoptosis. Objectives: The purpose of this research was to investigate the simultaneously effect of Valproic acid and gamma radiation on telomerase activity and bax and Bcl-2 protein level in MCF-7 breast cancer cell line. Materials and Methods: MCF-7 cells was treated with different dose of Valproic acid (0, 2, 8 and 16 mM/l) and single dose of gamma radiation (4 Gy/min. (cell toxicity was determined using neutral uptake test. Telomerase activity was determined using TRAP assay (PCR-ELISA) method. Bax and Bcl-2 protein level was determined by ELISA method, as well. Results: Combination of Valproic acid and gamma radiation increased significantly cell toxicity in a time and dose dependent manner compared with control (P < 0.0001). The ratio of Bax/Bcl-2 was increased in a dose dependent manner at 48 and 72 hour treatment (P < 0.0001). There was a decrease in Telomerase activity after 24, 48 and 72 hours treatment in a dose dependent manner (P < 0.0001). Conclusions: The increasing cell toxicity, apoptosis-inducing effects and decreasing telomerase activity may play an important role in the Valproic acid and radiation mechanism. The current survey suggested that it is likely beneficial to combine Valproic acid and gamma radiation to treat breast cancer. © 2015, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: protein Bax; protein bcl 2; telomerase; valproic acid, Article; breast cancer; breast cancer cell line; controlled study; cytotoxicity; enzyme activity; enzyme linked immunosorbent assay; gamma radiation; MCF 7 cell line; medical research; polymerase chain reaction; telomeric repeat amplification protocol
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Sep 2016 07:03
Last Modified: 14 Jan 2017 08:59
URI: http://eprints.goums.ac.ir/id/eprint/4761

Actions (login required)

View Item View Item