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Identification of novel genes involved in gastric carcinogenesis by suppression subtractive hybridization

Mottaghi-Dastjerdi, N. and Soltany-Rezaee-Rad, M. and Sepehrizadeh, Z. and Roshandel, G. and Ebrahimifard, F. and Setayesh, N. (2015) Identification of novel genes involved in gastric carcinogenesis by suppression subtractive hybridization. Human and Experimental Toxicology, 34 (1). pp. 3-11.

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Gastric cancer (GC) is one of the most common and life-threatening types of malignancies. Identification of the differentially expressed genes in GC is one of the best approaches for establishing new diagnostic and therapeutic targets. Furthermore, these investigations could advance our knowledge about molecular biology and the carcinogenesis of this cancer. To screen for the overexpressed genes in gastric adenocarcinoma, we performed suppression subtractive hybridization (SSH) on gastric adenocarcinoma tissue and the corresponding normal gastric tissue, and eight genes were found to be overexpressed in the tumor compared with those of the normal tissue. The genes were ribosomal protein L18A, RNase H2 subunit B, SEC13, eukaryotic translation initiation factor 4A1, tetraspanin 8, cytochrome c oxidase subunit 2, NADH dehydrogenase subunit 4, and mitochondrially encoded ATP synthase 6. The common functions among the identified genes include involvement in protein synthesis, involvement in genomic stability maintenance, metastasis, metabolic improvement, cell signaling pathways, and chemoresistance. Our results provide new insights into the molecular biology of GC and drug discovery: each of the identified genes could be further investigated as targets for prognosis evaluation, diagnosis, treatment, evaluation of the response to new anticancer drugs, and determination of the molecular pathogenesis of GC. © The Author(s) 2014.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: antineoplastic agent; cytochrome c oxidase subunit 2; eukaryotic translation initiation factor 4a1; l18a protein; mitochondrially encoded atp synthase 6; reduced nicotinamide adenine dinucleotide dehydrogenase subunit 4; ribosome protein; rnase h2 subunit b protein; sec13 protein; tetraspanin 8; unclassified drug; carrier protein; cytochrome c oxidase; cytochrome C oxidase subunit II; initiation factor 4A; MT-ATP6 protein, human; NADH dehydrogenase subunit 4; proton transporting adenosine triphosphate synthase; reduced nicotinamide adenine dinucleotide dehydrogenase; ribonuclease H; ribonuclease HII; ribosomal protein L18; ribosome protein; SEC13 protein, human; tetraspanin; TSPAN8 protein, human, adult; Article; cancer genetics; cancer prognosis; cancer resistance; comparative study; drug response; gene expression profiling; genomic instability; human; human tissue; male; middle aged; molecular pathology; protein synthesis; RNA extraction; RNA isolation; signal transduction; stomach adenocarcinoma; stomach cancer; suppression subtractive hybridization; adenocarcinoma; gene expression regulation; genetics; stomach tumor; subtractive hybridization, Eukaryota, Adenocarcinoma; Carrier Proteins; Electron Transport Complex IV; Eukaryotic Initiation Factor-4A; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Male; Middle Aged; Mitochondrial Proton-Translocating ATPases; NADH Dehydrogenase; Ribonuclease H; Ribosomal Proteins; Stomach Neoplasms; Subtractive Hybridization Techniques; Tetraspanins
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Sep 2016 06:43
Last Modified: 09 Jan 2017 07:59
URI: http://eprints.goums.ac.ir/id/eprint/4749

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