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The correlation between telomerase activity and BaX/BcL-2 ratio in valproic acid-treated MCF-7 breast cancer cell line

Vafaiyan, Z. and Gharaei, R. and Asadi, J. (2015) The correlation between telomerase activity and BaX/BcL-2 ratio in valproic acid-treated MCF-7 breast cancer cell line. Iranian Journal of Basic Medical Sciences, 18 (7). pp. 700-704.

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Objective(s): Valproic acid (VPA), a drug used in the treatment of neurological disorders, has been shown to have cytotoxic effects on cancer cells through different mechanisms. Telomerase, a ribonucleoprotein reverse transcriptase, is responsible for elongation of the telomere and is activated in cancers. A relation between telomerase activity and resistance to apoptosis has been established. This study focused on probable effects of VPA on MCF-7 cancer cells. In particular, we investigated VPA effects on viability, apoptosis and telomerase activity. Materials and Methods: Cytotoxicity effects of VPA on MCF-7 cells were determined by neutral red uptake assay. Cells were treated with different concentrations of VPA (0-32 mM) and telomerase activity and Bax and Bcl-2 protein levels were determined using TRAP assay (PCR-ELISA) method and ELISA method, respectively. Results: The cytotoxic effects of different concentration of VPA on MCF-7 cells were observed as a reduction in cell viability and telomerase activity and altered expression of Bcl-2 family protein levels. The results also showed that there is a significant correlation between reduction of telomerase activity and increase in Bax/Bcl-2 ratio (P=0.001). Conclusion: Our study demonstrated that cell viability of MCF-7 cells was decreased after treatment with VPA, probably through a reduction of telomerase activity and an increase in Bax/bcl-2 ratio. Therefore, it could be concluded that VPA is a potent anti-cancer agent for breast cancer cells through inhibition of telomerase activity and induction of apoptosis. © 2015, Mashhad University of Medical Sciences. All rights reserved.

Item Type: Article
Additional Information: cited By 2
Uncontrolled Keywords: neutral red; protein Bax; protein bcl 2; telomerase; valproic acid, apoptosis; Article; breast cancer; cell growth; cell viability; controlled study; cytotoxicity; drug sensitivity; enzyme activity; enzyme linked immunosorbent assay; human; human cell; LC50; MCF 7 cell line; microplate reader; polymerase chain reaction; protein expression; spectrophotometry; telomeric repeat amplification protocol
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Sep 2016 06:23
Last Modified: 16 Jan 2017 18:27
URI: http://eprints.goums.ac.ir/id/eprint/4740

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