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Correlation between hepatitis B G1896A precore mutations and HBeAg in chronic HBV patients

Zhand, S. and Karami, C. and Adli, A.H. and Tabarraei, A. and Khodabakhshi, B. and Moradi, A. (2015) Correlation between hepatitis B G1896A precore mutations and HBeAg in chronic HBV patients. Jundishapur Journal of Microbiology, 8 (2).

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Abstract

Background: Hepatitis B virus (HBV) infection is an important health concern worldwide, with critical outcomes. Hepatitis B e antigen (HBeAg) negative chronic hepatitis B is frequently caused by a mutation (G1896A) in the hepatitis B virus (HBV) precore (PC) reading frame, which creates a stop codon, causing premature termination of the HBe protein. Objectives: This study aimed to investigate the G1896A PC mutation and its effect on HBeAg detection in chronic HBV patients. Patients and Methods: In this study, 120 chronic HBV patients neither vaccinated or who had benefited from immunoglobulin therapy, were recruited. The HBV-DNA was extracted from plasma and polymerase chain reaction (PCR) was performed. Positive PCR products were subjected to automated sequencing. The HBV serological markers hepatitis B s antigen (HBsAg), HBeAg were tested. Results: One hundred out of 120 (83.3%) patients were HBeAg negative and 100% were HBsAg positive. The comparison of nucleotide sequences with the reference sequence (Accession number: AB033559) in HBeAg negative patients showed that there was a high rate of mutations in G1896A (93.18%). Conclusions: This study indicates that the rate of G1896A mutation at the PC region among HBeAg negative patients, in the Golestan province of Iran, was similar to the average rate encountered in other parts of Iran. The PC stop codon mutation was detected in 93.18% of HBeAg negative patients. Further studies with larger sample sizes are required to elucidate the exact role of these mutations in the clinical course of chronic HBV infection. © 2015, Ahvaz Jundishapur University of Medical Sciences.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: adenine; guanine; hepatitis B surface antigen; hepatitis B(e) antigen, adult; antigen detection; Article; controlled study; DNA extraction; female; genetic correlation; hepatitis B; Hepatitis B virus; human; Iran; major clinical study; male; nonhuman; nucleotide sequence; polymerase chain reaction; serology; stop codon; virus mutation, Hepatitis B virus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Sep 2016 05:20
Last Modified: 24 Dec 2016 09:00
URI: http://eprints.goums.ac.ir/id/eprint/4710

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