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Prevalence of Panton-valentine gene in Staphylococcus aureus isolated from clinical samples and healthy carriers in Gorgan city, north of Iran

Kamarehei, F. and Rahimi-Alang, S. and Vaez, H. and Ghaemi, E. (2015) Prevalence of Panton-valentine gene in Staphylococcus aureus isolated from clinical samples and healthy carriers in Gorgan city, north of Iran. Minerva Biotecnologica, 27 (1). pp. 51-54.

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Abstract

Aim. Staphylococcus aureus (S. aureus) is a nosocomial and community acquired pathogen. S. aureus is a pathogen that causes several types of disease from skin infections to systemic diseases that is because of having several virulence factors such as enzymes, toxins, superantigens and Panton-Valentine leukocidin (pvl). pvl is a bi-component leukotoxin that destroy PMNs and monocytes and causes furunculosis, abscesses and necrotizing soft tissue infections in patients without any risk factors for such infections. The goal of this study was determine the prevalence of pvl gene in S. aureus isolated from patients and healthy carriers in Gorgan city, north of Iran. Methods. One hundred seventy isolates of S. aureus, 95 from patients and 75 healthy carriers, were collected during one year. After identification and purification, DNA extraction was done by phenol-chloroform method. Amplification of pvl gene was done by specific primer and polymerase chain reaction method. Results. Among the 170 isolates of S. aureus, 20 contained pvl gene. The frequency of isolates contained pvl gene in MRSA and MSSA isolates were 21.6, 19.3, which was not statistically significant. The frequency of these genes was not related to age, sex and source of isolation from patients. Conclusion. The frequency of pvl gene in this region were much higher than expected. © Copyright 2016 Edizioni Minerva Medica.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: chloroform; Panton Valentine leukocidin; phenol, adult; age distribution; Article; bacterium identification; bacterium isolation; biochemical analysis; controlled study; DNA extraction; female; gene amplification; gene frequency; genetic analysis; human; Iran; major clinical study; male; methicillin resistant Staphylococcus aureus; methicillin susceptible Staphylococcus aureus; polymerase chain reaction; prevalence; protein analysis; protein purification; sex allocation; Staphylococcus aureus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Sep 2016 04:55
Last Modified: 16 Jan 2017 08:39
URI: http://eprints.goums.ac.ir/id/eprint/4692

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