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Downregulation of plasma MiR-142-3p and MiR-26a-5p in patients with colorectal carcinoma

Ghanbari, R. and Mosakhani, N. and Asadi, J. and Nouraee, N. and Mowla, S.J. and Yazdani, Y. and Mohamadkhani, A. and Poustchi, H. and Knuutila, S. and Malekzadeh, R. (2015) Downregulation of plasma MiR-142-3p and MiR-26a-5p in patients with colorectal carcinoma. Iranian Journal of Cancer Prevention, 8 (3).

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Abstract

Background: Colorectal cancer is one of the most commonly diagnosed cancers and cancer- related death worldwide. Identification of new specific biomarkers could be helpful to detection of this malignancy. Altered plasma microRNA expression has been identified in many cancers, including colorectal cancer. Objectives: The main objective of this study was to identify the circulating microRNAs with the most expression changes in colorectal cancer patients compared with neoplasm free healthy individuals. Materials and Methods: MicroRNA expression profiling was performed on plasma samples of 37 colorectal cancer patients and 8 normal subjects using microRNA microarray. Quantitative real-time reverse transcription polymerase chain reaction was used to validate the two selected altered microR NAs. Plasma samples from 61 colorectal cancer patients and 24 normal subjects were used in our validation study. Results: In profiling study we found a panel of six plasma microRNAs with significant downregulation. MicroRNA-142-3p and microRNA-26a-5p were selected and validated by polymerase chain reaction. Our results demonstrated that expression levels of plasma microRNA-142-3p and microRNA-26a-5p were significantly downregulated in patients with colorectal cancer when compared to control group. Conclusions: Our findings suggest that downregulation of plasma microRNA-142-3p and microRNA-26a-5p might serve as novel noninvasive biomarkers in the diagnosis of colorectal cancer, although more studies are needed to highlight the theoretical strengths. © 2015, Iranian Journal of Cancer Prevention.

Item Type: Article
Additional Information: cited By 2
Uncontrolled Keywords: microRNA; microRNA 142 3p; microRNA 26a 5p; microRNA 27a 3p; microRNA 326; microRNA 331 3p; unclassified drug, adult; Article; blood sampling; clinical article; colorectal carcinoma; controlled study; diagnostic value; disease marker; female; gene expression profiling; gene expression regulation; human; male; microarray analysis; quantitative analysis; real time polymerase chain reaction; reverse transcription polymerase chain reaction; tumor localization; validation process
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Sep 2016 03:40
Last Modified: 15 Jan 2017 10:19
URI: http://eprints.goums.ac.ir/id/eprint/4687

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